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- Blot_(biology) abstract "A blot, in molecular biology and genetics, is a method of transferring proteins, DNA or RNA, onto a carrier (for example, a nitrocellulose PVDF or nylon membrane). In many instances, this is done after a gel electrophoresis, transferring the molecules from the gel onto the blotting membrane, and other times adding the samples directly onto the membrane. After the blotting, the transferred proteins, DNA or RNA are then visualized by colorant staining (for example, silver staining of proteins), autoradiographic visualization of radioactive labelled molecules (performed before the blot), or specific labelling of some proteins or nucleic acids. The latter is done with antibodies or hybridization probes that bind only to some molecules of the blot and have an enzyme joined to them. After proper washing, this enzymatic activity (and so, the molecules we search in the blot) is visualized by incubation with proper reactive, rendering either a colored deposit on the blot or a chemiluminiscent reaction which is registered by photographic film.".
- Blot_(biology) wikiPageID "770314".
- Blot_(biology) wikiPageRevisionID "590799479".
- Blot_(biology) hasPhotoCollection Blot_(biology).
- Blot_(biology) subject Category:Laboratory_techniques.
- Blot_(biology) subject Category:Molecular_biology.
- Blot_(biology) type Ability105616246.
- Blot_(biology) type Abstraction100002137.
- Blot_(biology) type Cognition100023271.
- Blot_(biology) type Know-how105616786.
- Blot_(biology) type LaboratoryTechniques.
- Blot_(biology) type Method105660268.
- Blot_(biology) type PsychologicalFeature100023100.
- Blot_(biology) type Technique105665146.
- Blot_(biology) comment "A blot, in molecular biology and genetics, is a method of transferring proteins, DNA or RNA, onto a carrier (for example, a nitrocellulose PVDF or nylon membrane). In many instances, this is done after a gel electrophoresis, transferring the molecules from the gel onto the blotting membrane, and other times adding the samples directly onto the membrane.".
- Blot_(biology) label "Blot (biology)".
- Blot_(biology) label "Blotting DNA".
- Blot_(biology) label "Blotting".
- Blot_(biology) label "Transfert (biologie moléculaire)".
- Blot_(biology) label "Блоттинг".
- Blot_(biology) sameAs Blotting.
- Blot_(biology) sameAs Transfert_(biologie_moléculaire).
- Blot_(biology) sameAs Blotting_DNA.
- Blot_(biology) sameAs m.03b22t.
- Blot_(biology) sameAs Q885458.
- Blot_(biology) sameAs Q885458.
- Blot_(biology) sameAs Blot_(biology).
- Blot_(biology) wasDerivedFrom Blot_(biology)?oldid=590799479.
- Blot_(biology) isPrimaryTopicOf Blot_(biology).