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- Dilution_cloning abstract "Dilution cloning or cloning by limiting dilution describes a procedure to obtain a monoclonal cell population starting from a polyclonal mass of cells.This is achieved by setting up a series of increasing dilutions of the parent (polyclonal) cell culture. A suspension of the parent cells is made. Appropriate dilutions are then made, depending on cell number in the starting population, as well as the viability and characteristics of the cells being cloned.After the final dilutions are produced, aliquots of the suspension are plated or placed in wells and incubated. If all works correctly, a monoclonal cell colony will be produced. Applications for the procedure include cloning of parasites, T cells, transgenic cells, and macrophages.".
- Dilution_cloning wikiPageExternalLink Cloning%20Lab.pdf.
- Dilution_cloning wikiPageExternalLink 3-2Single_cell_cloning_protocol.pdf.
- Dilution_cloning wikiPageExternalLink bq072e.pdf.
- Dilution_cloning wikiPageExternalLink PMC2589791.
- Dilution_cloning wikiPageExternalLink Cloning-by-Limiting-Dilution-of-Hybridoma-86.html.
- Dilution_cloning wikiPageExternalLink cbld.htm.
- Dilution_cloning wikiPageID "6885971".
- Dilution_cloning wikiPageRevisionID "602341263".
- Dilution_cloning hasPhotoCollection Dilution_cloning.
- Dilution_cloning subject Category:Molecular_biology.
- Dilution_cloning comment "Dilution cloning or cloning by limiting dilution describes a procedure to obtain a monoclonal cell population starting from a polyclonal mass of cells.This is achieved by setting up a series of increasing dilutions of the parent (polyclonal) cell culture. A suspension of the parent cells is made.".
- Dilution_cloning label "Dilution cloning".
- Dilution_cloning label "Limiting Dilution Cloning".
- Dilution_cloning sameAs Limiting_Dilution_Cloning.
- Dilution_cloning sameAs m.0gv8wj.
- Dilution_cloning sameAs Q5277084.
- Dilution_cloning sameAs Q5277084.
- Dilution_cloning wasDerivedFrom Dilution_cloning?oldid=602341263.
- Dilution_cloning isPrimaryTopicOf Dilution_cloning.