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- Secondary_plot_(kinetics) abstract "In enzyme kinetics, a secondary plot uses the intercept or slope from several Lineweaver-Burk plots to find additional kinetic constants.For example, when a set of v by [S] curves from an enzyme with a ping–pong mechanism (varying substrate A, fixed substrate B) are plotted in a Lineweaver–Burk plot, a set of parallel lines will be produced.The following Michaelis–Menten equation relates the initial reaction rate v0 to the substrate concentrations [A] and [B]:The y-intercept of this equation is equal to the following:The y-intercept is determined at several different fixed concentrations of substrate B (and varying substrate A). The y-intercept values are then plotted versus 1/[B] to determine the Michaelis constant for substrate B, , as shown in the Figure to the right. The slope is equal to divided by and the intercept is equal to 1 over .".
- Secondary_plot_(kinetics) thumbnail Secondary_Plot.jpg?width=300.
- Secondary_plot_(kinetics) wikiPageID "26994195".
- Secondary_plot_(kinetics) wikiPageRevisionID "547094151".
- Secondary_plot_(kinetics) hasPhotoCollection Secondary_plot_(kinetics).
- Secondary_plot_(kinetics) subject Category:Enzyme_kinetics.
- Secondary_plot_(kinetics) comment "In enzyme kinetics, a secondary plot uses the intercept or slope from several Lineweaver-Burk plots to find additional kinetic constants.For example, when a set of v by [S] curves from an enzyme with a ping–pong mechanism (varying substrate A, fixed substrate B) are plotted in a Lineweaver–Burk plot, a set of parallel lines will be produced.The following Michaelis–Menten equation relates the initial reaction rate v0 to the substrate concentrations [A] and [B]:The y-intercept of this equation is equal to the following:The y-intercept is determined at several different fixed concentrations of substrate B (and varying substrate A). ".
- Secondary_plot_(kinetics) label "Secondary plot (kinetics)".
- Secondary_plot_(kinetics) sameAs m.0bs2c8x.
- Secondary_plot_(kinetics) sameAs Q7443864.
- Secondary_plot_(kinetics) sameAs Q7443864.
- Secondary_plot_(kinetics) wasDerivedFrom Secondary_plot_(kinetics)?oldid=547094151.
- Secondary_plot_(kinetics) depiction Secondary_Plot.jpg.
- Secondary_plot_(kinetics) isPrimaryTopicOf Secondary_plot_(kinetics).
