Matches in UGent Biblio for { ?s ?p A prominent trend which has been observed in recent years in the analysis of veterinary drugs and growth-promoting agents is the shift from target-oriented procedures, mainly based on liquid chromatography coupled to triple-quadrupole mass spectrometry (LC-QqQ-MS), towards accurate mass full scan MS (such as time of flight (ToF) and Fourier Transform (FT) MS). In a previous study the applicability of HR-Orbitrap-MS for confirmatory analysis of growth-promoting agents in meat was compared to that of a QqQ-MS. Validation according to CD 2002/657/EC demonstrated that steroid analysis based on single stage Orbitrap mass spectrometry, operating at a resolution of 50,000 FWHM, is indeed capable to compete with QqQ-MS in terms of selectivity/specificity, while providing excellent linearity (for most compounds > 0.99) but somewhat inferior sensitivity. Therefore our goal was to investigate the capability of a TripleTOF™ (QqTOF) instrument to provide sensitive high resolution accurate mass MS data (> 25,000 FWHM) but at the same time MS/MS data at high acquisitions speeds. For the development of the detection method the fast Information Dependent Acquisition (IDA) combining a TOF-MS survey scan followed by dependent TOF-MS/MS scans will be compared to the more time consuming workflow of optimizing the collision energy per compound. Afterwards a validation according to the CD/2002/657/EC will be performed, in this way the accurate mass MS data is used to accurately quantify the targeted steroids and the additionally collected MS/MS spectra are used to confidently identify the compounds. The benefit of this instrument compared to the QqQ-MS is the ability to mine the data retrospectively for identifying non-targeted and unexpected compounds, which is beneficiary in the light of the national control plan of growth-promoting agents. When compared to the single stage Orbitrap, a lower resolution is obtained, however due to the acquired MS/MS data the TripleTOF™ is expected to provide an advantage especially for those compounds (i.e. some estrogens) for which the threshold of 20% of repeatability and intra-laboratory reproducibility, correlated to an inferior precision, was exceeded with the single stage Orbitrap. Finally, a thorough comparison and conclusion will be provided for the detection of anabolic steroids in meat by means of the TripleTOF™-MS in terms of linearity, precision, sensitivity and other relevant performance characteristics, as the validation is in progress.. }
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- aggregation abstract "A prominent trend which has been observed in recent years in the analysis of veterinary drugs and growth-promoting agents is the shift from target-oriented procedures, mainly based on liquid chromatography coupled to triple-quadrupole mass spectrometry (LC-QqQ-MS), towards accurate mass full scan MS (such as time of flight (ToF) and Fourier Transform (FT) MS). In a previous study the applicability of HR-Orbitrap-MS for confirmatory analysis of growth-promoting agents in meat was compared to that of a QqQ-MS. Validation according to CD 2002/657/EC demonstrated that steroid analysis based on single stage Orbitrap mass spectrometry, operating at a resolution of 50,000 FWHM, is indeed capable to compete with QqQ-MS in terms of selectivity/specificity, while providing excellent linearity (for most compounds > 0.99) but somewhat inferior sensitivity. Therefore our goal was to investigate the capability of a TripleTOF™ (QqTOF) instrument to provide sensitive high resolution accurate mass MS data (> 25,000 FWHM) but at the same time MS/MS data at high acquisitions speeds. For the development of the detection method the fast Information Dependent Acquisition (IDA) combining a TOF-MS survey scan followed by dependent TOF-MS/MS scans will be compared to the more time consuming workflow of optimizing the collision energy per compound. Afterwards a validation according to the CD/2002/657/EC will be performed, in this way the accurate mass MS data is used to accurately quantify the targeted steroids and the additionally collected MS/MS spectra are used to confidently identify the compounds. The benefit of this instrument compared to the QqQ-MS is the ability to mine the data retrospectively for identifying non-targeted and unexpected compounds, which is beneficiary in the light of the national control plan of growth-promoting agents. When compared to the single stage Orbitrap, a lower resolution is obtained, however due to the acquired MS/MS data the TripleTOF™ is expected to provide an advantage especially for those compounds (i.e. some estrogens) for which the threshold of 20% of repeatability and intra-laboratory reproducibility, correlated to an inferior precision, was exceeded with the single stage Orbitrap. Finally, a thorough comparison and conclusion will be provided for the detection of anabolic steroids in meat by means of the TripleTOF™-MS in terms of linearity, precision, sensitivity and other relevant performance characteristics, as the validation is in progress.".