Matches in UGent Biblio for { ?s ?p Bananas (Musa spp.), which constitute a staple food in the Great Lakes region of east and central Africa, are characterized by a long growth cycle of 17-22 months. This long cropping cycle affects the crop's productivity per unit time and if reduced has the potential to increase yield. However, lack of early maturity genes in the Musa germplasm and the biology of banana impede the application of conventional breeding for this trait. In this study, we investigated the genetic transformation strategy of over-expressing D-type cyclin genes that have been shown to shorten cell cycle duration and increase growth in model plant species. Using the Agrobacterium tumefaciens transformation technique, embryogenic cells of banana cv. 'Sukalindiizi' (AAB) were transformed with the Arabidopsis thaliana D2;1 gene under the control of maize ubiquitin promoter and neomycin phosphotransferase (nptII) as the selectable marker gene. Selection of the transformed cells on neomycin supplemented medium gave a regeneration frequency of 15% and transformation frequency of 46.8%. PCR, Southern blot and reverse transcriptase PCR analyses on the regenerated shoots confirmed the presence, integration and transcription of the transgene, respectively. Preliminary growth evaluation of glasshouse-potted regenerants identified improved leaf elongation in two lines. Detailed morphological analysis of the transgenic lines is ongoing.. }
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- aggregation abstract "Bananas (Musa spp.), which constitute a staple food in the Great Lakes region of east and central Africa, are characterized by a long growth cycle of 17-22 months. This long cropping cycle affects the crop's productivity per unit time and if reduced has the potential to increase yield. However, lack of early maturity genes in the Musa germplasm and the biology of banana impede the application of conventional breeding for this trait. In this study, we investigated the genetic transformation strategy of over-expressing D-type cyclin genes that have been shown to shorten cell cycle duration and increase growth in model plant species. Using the Agrobacterium tumefaciens transformation technique, embryogenic cells of banana cv. 'Sukalindiizi' (AAB) were transformed with the Arabidopsis thaliana D2;1 gene under the control of maize ubiquitin promoter and neomycin phosphotransferase (nptII) as the selectable marker gene. Selection of the transformed cells on neomycin supplemented medium gave a regeneration frequency of 15% and transformation frequency of 46.8%. PCR, Southern blot and reverse transcriptase PCR analyses on the regenerated shoots confirmed the presence, integration and transcription of the transgene, respectively. Preliminary growth evaluation of glasshouse-potted regenerants identified improved leaf elongation in two lines. Detailed morphological analysis of the transgenic lines is ongoing.".