Matches in UGent Biblio for { ?s ?p DNA was delivered to intact embryonic axes of the legume Phaseolus vulgaris L. through electroporation. Expression of the beta-glucuronidase reporter gene was observed in hypocotyl and epicotyl tissue in a spot-like manner. Transgene expression was high when a single pulse of 260 ms at a field strength of 225 V . cm(-1) was applied but could be achieved within a wide range of electrical conditions. Linearization of plasmid DNA greatly enhanced transient expression levels. The procedure was successful for embryonic axes of all tested P. vulgaris cultivars, for similar explants of several large-seeded leguminous species, as well as for some other tissues of P. vulgaris.. }
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- aggregation abstract "DNA was delivered to intact embryonic axes of the legume Phaseolus vulgaris L. through electroporation. Expression of the beta-glucuronidase reporter gene was observed in hypocotyl and epicotyl tissue in a spot-like manner. Transgene expression was high when a single pulse of 260 ms at a field strength of 225 V . cm(-1) was applied but could be achieved within a wide range of electrical conditions. Linearization of plasmid DNA greatly enhanced transient expression levels. The procedure was successful for embryonic axes of all tested P. vulgaris cultivars, for similar explants of several large-seeded leguminous species, as well as for some other tissues of P. vulgaris.".