Matches in UGent Biblio for { ?s ?p In this study, a generic extraction protocol and full-scan high-resolution Orbitrap-MS detection method were developed, enabling the metabolomic screening for carotenoids in tomato fruit tissue. To this end, the carotenoids lutein, zeaxanthin, α-carotene, β-carotene and lycopene (representing both xanthofylls and carotenes) were considered. The extraction procedure was optimized by means of a D-optimal design and consisted of a liquid-liquid extraction with methanol/methyl-tert-butylether (1:1, v/v). The considered compounds were detected by a single-stage ExactiveTM mass spectrometer, operating at a mass resolution of 100,000 full width at half maximum. The validation study demonstrated excellent performance in terms of linearity (R2 > 0.99), repeatability (CV ≤ 10.6%), within-laboratory reproducibility (CV ≤ 12.2%), and mean corrected recovery (ranging from 85 to 106%). Additionally, a comparative evaluation towards well-established detection techniques, i.e. MS/MS and UV-VIS PDA, indicated superior performance of high-resolution Orbitrap-MS with regard to specificity/selectivity and sensitivity (with LODs ranging from 1.0 to 3.8 pg μL-1). As a result, it may be concluded that high-resolution Orbitrap-MS is a suited alternative for UV-VIS or MS/MS in analyzing carotenoids and may offer significant value in carotenoid research because of the metabolomic screening possibilities.. }
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- aggregation abstract "In this study, a generic extraction protocol and full-scan high-resolution Orbitrap-MS detection method were developed, enabling the metabolomic screening for carotenoids in tomato fruit tissue. To this end, the carotenoids lutein, zeaxanthin, α-carotene, β-carotene and lycopene (representing both xanthofylls and carotenes) were considered. The extraction procedure was optimized by means of a D-optimal design and consisted of a liquid-liquid extraction with methanol/methyl-tert-butylether (1:1, v/v). The considered compounds were detected by a single-stage ExactiveTM mass spectrometer, operating at a mass resolution of 100,000 full width at half maximum. The validation study demonstrated excellent performance in terms of linearity (R2 > 0.99), repeatability (CV ≤ 10.6%), within-laboratory reproducibility (CV ≤ 12.2%), and mean corrected recovery (ranging from 85 to 106%). Additionally, a comparative evaluation towards well-established detection techniques, i.e. MS/MS and UV-VIS PDA, indicated superior performance of high-resolution Orbitrap-MS with regard to specificity/selectivity and sensitivity (with LODs ranging from 1.0 to 3.8 pg μL-1). As a result, it may be concluded that high-resolution Orbitrap-MS is a suited alternative for UV-VIS or MS/MS in analyzing carotenoids and may offer significant value in carotenoid research because of the metabolomic screening possibilities.".