Matches in UGent Biblio for { ?s ?p The conditions of thin-layer chromatography separation of related aflatoxins and zearalenones in the presence of beta-cyclodextrin and 2-hydroxypropyl-beta-cyclodextrin were studied. Effects of the stationary phase and mobile phase composition were investigated. Analytical conditions for the separation and simultaneous semi-quantitative fluorescence detection of aflatoxins B(1), B(2), G(1) and G(2), zearalenone and alpha-zearalenol on normal-phase plates (silica gel, polyamide) and reversed-phase plates (C18) with cyclodextrin modified mobile phase were optimised. The limit of quantification was found 2 ng per spot for aflatoxin G(1) and aflatoxin B(2), 3.5 ng for aflatoxin B(1) and aflatoxin G(2) and 100 ng per spot for zearalenone and alpha-zearalenol. Addition of cyclodextrins to the mobile phase allowed a decrease in the amount of toxic solvents, and improved separation characteristics, but did not improve the limit of quantification.. }
Showing items 1 to 1 of
1
with 100 items per page.
- aggregation abstract "The conditions of thin-layer chromatography separation of related aflatoxins and zearalenones in the presence of beta-cyclodextrin and 2-hydroxypropyl-beta-cyclodextrin were studied. Effects of the stationary phase and mobile phase composition were investigated. Analytical conditions for the separation and simultaneous semi-quantitative fluorescence detection of aflatoxins B(1), B(2), G(1) and G(2), zearalenone and alpha-zearalenol on normal-phase plates (silica gel, polyamide) and reversed-phase plates (C18) with cyclodextrin modified mobile phase were optimised. The limit of quantification was found 2 ng per spot for aflatoxin G(1) and aflatoxin B(2), 3.5 ng for aflatoxin B(1) and aflatoxin G(2) and 100 ng per spot for zearalenone and alpha-zearalenol. Addition of cyclodextrins to the mobile phase allowed a decrease in the amount of toxic solvents, and improved separation characteristics, but did not improve the limit of quantification.".