Matches in Ghent University Academic Bibliography for { <https://biblio.ugent.be/publication/01HACG4GAGK0MGJDPC3EZ4PCYF> ?p ?o. }
Showing items 1 to 23 of
23
with 100 items per page.
- 01HACG4GAGK0MGJDPC3EZ4PCYF classification A1.
- 01HACG4GAGK0MGJDPC3EZ4PCYF date "1982".
- 01HACG4GAGK0MGJDPC3EZ4PCYF language "eng".
- 01HACG4GAGK0MGJDPC3EZ4PCYF type journalArticle.
- 01HACG4GAGK0MGJDPC3EZ4PCYF hasPart 01HACG78FZ541RV32H9Y160VYA.pdf.
- 01HACG4GAGK0MGJDPC3EZ4PCYF subject "Biology and Life Sciences".
- 01HACG4GAGK0MGJDPC3EZ4PCYF subject "Chemistry".
- 01HACG4GAGK0MGJDPC3EZ4PCYF doi "10.1111/j.1432-1033.1982.tb06654.x".
- 01HACG4GAGK0MGJDPC3EZ4PCYF issn "0014-2956".
- 01HACG4GAGK0MGJDPC3EZ4PCYF issue "1".
- 01HACG4GAGK0MGJDPC3EZ4PCYF volume "125".
- 01HACG4GAGK0MGJDPC3EZ4PCYF abstract "The cell wall of leprosy-derived corynebacteria (a group of ‘diphtheroids’ isolated from human leprosy lesions and patients' blood) was previously shown to contain, in addition to peptidoglycan and arabinogalactan, mycolic acids. These α-branched β-hydroxy fatty acids were attributed to the corynomycolic group, according to their RF in monodimensional thin-layer chromatography. In the present work, mycolic acids from leprosy-derived and reference corynebacteria have been fractionated by monodimensional and bidimensional thin-layer chro- matography and by gas chromatography. Pyrolyzed mycolic acids have been analysed on conventional packed columns, whereas intact methyl esters of mycolic acids with free and silylated β-hydroxyl group have been analyzed on capillary columns, and their structure has been established by mass spectrometry. In all leprosy-derived corynebacteria, some 20 components containing 24- 36 carbon atoms and 0-4 double bonds were obtained. The three major groups had 32, 34 and 36 carbons, and the frequency of unsaturated versus saturated chains increased proportionally to the molecular weight. For comparison, the main components of a reference corynebacterium, Corynebacterium diphtheriae PW8, had 30 and 32 carbons, and their hydro- carbon chains were essentially saturated. This work confirms the relative chemical homogeneity of different leprosy-derived corynebacteria and describes some peculiar traits in the chemical structure of this group of organisms. In addition, it shows the complexity of the mycolic acid fraction of corynebacterial cell wall and suggests that the mycolic acid pattern is a sort of fingerprint of each bacterial strain grown under standard conditions. Finally, the fractionation of intact corynomycolic acid methyl esters with free or silylated β-hydroxyl group by capillary gas chromatography proved to be the best analytical procedure at present available for resolving this complex mixture of corynomycolate isomers. Structural determination of silylated samples by mass spectrometry is preferred because they have more diagnostic fragments.".
- 01HACG4GAGK0MGJDPC3EZ4PCYF author F3A39052-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HACG4GAGK0MGJDPC3EZ4PCYF author d4af6cd7-cc00-40ae-b261-d3eb4fd3e84b.
- 01HACG4GAGK0MGJDPC3EZ4PCYF author urn:uuid:5be2e358-d686-4cf3-acdb-9ed5a97f4d21.
- 01HACG4GAGK0MGJDPC3EZ4PCYF author urn:uuid:eb4e63f4-777a-44dd-b70b-f46d1d5e1cc6.
- 01HACG4GAGK0MGJDPC3EZ4PCYF dateCreated "2023-09-15T13:22:56Z".
- 01HACG4GAGK0MGJDPC3EZ4PCYF dateModified "2024-10-29T16:20:59Z".
- 01HACG4GAGK0MGJDPC3EZ4PCYF name "Analysis of mycolic acids from a group of corynebacteria by capillary gas chromatography and mass spectrometry".
- 01HACG4GAGK0MGJDPC3EZ4PCYF pagination urn:uuid:7db355f0-e34b-424b-bcce-b43786307872.
- 01HACG4GAGK0MGJDPC3EZ4PCYF sameAs LU-01HACG4GAGK0MGJDPC3EZ4PCYF.
- 01HACG4GAGK0MGJDPC3EZ4PCYF sourceOrganization urn:uuid:0467b69f-a39b-429b-b116-c2eb0869e259.
- 01HACG4GAGK0MGJDPC3EZ4PCYF type A1.