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- ABTS abstract "In biochemistry, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) or ABTS is chemical compound used to observe the reaction kinetics of specific enzymes. A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect for binding of molecules to each other.It is commonly used as a substrate with hydrogen peroxide for a peroxidase enzyme or alone with blue multicopper oxidase enzymes such as laccase or bilirubin oxidase. Its use allows the reaction kinetics of peroxidases themselves to be followed. In this way it also can be used to indirectly follow the reaction kinetics of any hydrogen peroxide-producing enzyme, or to simply quantify the amount of hydrogen peroxide in a sample. The formal reduction potentials for ABTS are high enough for it to act as an electron donor for the reduction of oxo species such as molecular oxygen and hydrogen peroxide, particularly at the less-extreme pH values encountered in biological catalysis. Under these conditions, the sulfonate groups are fully deprotonated and the mediator exists as a dianion.ABTS–· + e– → ABTS2– E°′ = 0.67 V vs SHEABTS + e– → ABTS–· E°′ = 1.08 V vs SHEThis compound is chosen because the enzyme facilitates the reaction with hydrogen peroxide, turning it into a green and soluble end-product. Its new absorbance maximum of 420 nm light (ε = 3.6 × 104 M–1 cm–1) can easily be followed with a spectrophotometer, a common laboratory instrument. It is sometimes used as part of a glucose estimating reagent when finding glucose concentrations of solutions such as blood serum.ABTS is also frequently used by the food industry and agricultural researchers to measure the antioxidant capacities of foods. In this assay, ABTS is converted to its radical cation by addition of sodium persulfate. This radical cation is blue in color and absorbs light at 734 nm. The ABTS radical cation is reactive towards most antioxidants including phenolics, thiols and Vitamin C. During this reaction, the blue ABTS radical cation is converted back to its colorless neutral form. The reaction may be monitored spectrophotometrically. This assay is often referred to as the Trolox equivalent antioxidant capacity (TEAC) assay. The reactivity of the various antioxidants tested are compared to that of Trolox, which is a water-soluble analog of vitamin E.".
- ABTS iupacName "2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)".
- ABTS thumbnail ABTS.png?width=300.
- ABTS wikiPageID "2730088".
- ABTS wikiPageRevisionID "606343599".
- ABTS hasPhotoCollection ABTS.
- ABTS imagefile "ABTS.png".
- ABTS iupacname "22".
- ABTS verifiedrevid "399346050".
- ABTS subject Category:Biochemistry_methods.
- ABTS subject Category:Enzyme_kinetics.
- ABTS subject Category:Sulfonic_acids.
- ABTS type Ability105616246.
- ABTS type Abstraction100002137.
- ABTS type Acid114607521.
- ABTS type BiochemistryMethods.
- ABTS type Chemical114806838.
- ABTS type Cognition100023271.
- ABTS type Compound114818238.
- ABTS type Know-how105616786.
- ABTS type Material114580897.
- ABTS type Matter100020827.
- ABTS type Method105660268.
- ABTS type Part113809207.
- ABTS type PhysicalEntity100001930.
- ABTS type PsychologicalFeature100023100.
- ABTS type Relation100031921.
- ABTS type Substance100019613.
- ABTS type SulfonicAcid114616508.
- ABTS type SulfonicAcids.
- ABTS type ChemicalCompound.
- ABTS type ChemicalSubstance.
- ABTS type ChemicalSubstanceType.
- ABTS type ChemicalObject.
- ABTS type Thing.
- ABTS comment "In biochemistry, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) or ABTS is chemical compound used to observe the reaction kinetics of specific enzymes. A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect for binding of molecules to each other.It is commonly used as a substrate with hydrogen peroxide for a peroxidase enzyme or alone with blue multicopper oxidase enzymes such as laccase or bilirubin oxidase.".
- ABTS label "ABTS".
- ABTS label "ABTS".
- ABTS label "ABTS".
- ABTS sameAs ABTS.
- ABTS sameAs ABTS.
- ABTS sameAs m.07_hrx.
- ABTS sameAs Q287582.
- ABTS sameAs Q287582.
- ABTS sameAs ABTS.
- ABTS wasDerivedFrom ABTS?oldid=606343599.
- ABTS depiction ABTS.png.
- ABTS isPrimaryTopicOf ABTS.