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- Allele-specific_oligonucleotide abstract "An allele-specific oligonucleotide (ASO) is a short piece of synthetic DNA complementary to the sequence of a variable target DNA. It acts as a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler Dot blot assay. It is a common tool used in genetic testing, forensics, and Molecular Biology research.An ASO is typically an oligonucleotide of 15–21 nucleotide bases in length. It is designed (and used) in a way that makes it specific for only one version, or allele, of the DNA being tested. The length of the ASO, which strand it is chosen from, and the conditions by which it is bound to (and washed from) the target DNA all play a role in its specificity. These probes can usually be designed to detect a difference of as little as 1 base in the target's genetic sequence, a basic ability in the assay of single-nucleotide polymorphisms (SNPs), important in genotype analysis and the Human Genome Project. To be detected after it has bound to its target, the ASO must be labeled with a radioactive, enzymatic, or fluorescent tag. The Illumina Methylation Assay technology takes advantage of ASO to detect one base pair difference (cytosine versus thymine) to measure methylation at a specific CpG site.".
- Allele-specific_oligonucleotide thumbnail ASODemo.jpg?width=300.
- Allele-specific_oligonucleotide wikiPageExternalLink ASO_test_for_CF2.html.
- Allele-specific_oligonucleotide wikiPageID "10099472".
- Allele-specific_oligonucleotide wikiPageRevisionID "593430330".
- Allele-specific_oligonucleotide hasPhotoCollection Allele-specific_oligonucleotide.
- Allele-specific_oligonucleotide subject Category:DNA_profiling_techniques.
- Allele-specific_oligonucleotide subject Category:Laboratory_techniques.
- Allele-specific_oligonucleotide subject Category:Molecular_biology.
- Allele-specific_oligonucleotide subject Category:Molecular_biology_techniques.
- Allele-specific_oligonucleotide type Ability105616246.
- Allele-specific_oligonucleotide type Abstraction100002137.
- Allele-specific_oligonucleotide type Cognition100023271.
- Allele-specific_oligonucleotide type Know-how105616786.
- Allele-specific_oligonucleotide type LaboratoryTechniques.
- Allele-specific_oligonucleotide type Method105660268.
- Allele-specific_oligonucleotide type MolecularBiologyTechniques.
- Allele-specific_oligonucleotide type PsychologicalFeature100023100.
- Allele-specific_oligonucleotide type Technique105665146.
- Allele-specific_oligonucleotide comment "An allele-specific oligonucleotide (ASO) is a short piece of synthetic DNA complementary to the sequence of a variable target DNA. It acts as a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler Dot blot assay. It is a common tool used in genetic testing, forensics, and Molecular Biology research.An ASO is typically an oligonucleotide of 15–21 nucleotide bases in length.".
- Allele-specific_oligonucleotide label "Allele-specific oligonucleotide".
- Allele-specific_oligonucleotide sameAs m.02q1s6d.
- Allele-specific_oligonucleotide sameAs Q4731430.
- Allele-specific_oligonucleotide sameAs Q4731430.
- Allele-specific_oligonucleotide sameAs Allele-specific_oligonucleotide.
- Allele-specific_oligonucleotide wasDerivedFrom Allele-specific_oligonucleotide?oldid=593430330.
- Allele-specific_oligonucleotide depiction ASODemo.jpg.
- Allele-specific_oligonucleotide isPrimaryTopicOf Allele-specific_oligonucleotide.