Matches in DBpedia 2014 for { <http://dbpedia.org/resource/Amplified_fragment_length_polymorphism> ?p ?o. }
Showing items 1 to 39 of
39
with 100 items per page.
- Amplified_fragment_length_polymorphism abstract "AFLP-PCR or just AFLP is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by Keygene, AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments. A subset of the restriction fragments is then selected to be amplified. This selection is achieved by using primers complementary to the adaptor sequence, the restriction site sequence and a few nucleotides inside the restriction site fragments (as described in detail below). The amplified fragments are separated and visualized on denaturing polyacrylamide gels, either through autoradiography or fluorescence methodologies, or via automated capillary sequencing instruments.Although AFLP is commonly referred to as "Amplified fragment length polymorphism", the resulting data are not scored as length polymorphisms, but instead as presence-absence polymorphisms.AFLP-PCR is a highly sensitive method for detecting polymorphisms in DNA. The technique was originally described by Vos and Zabeau in 1993. In detail, the procedure of this technique is divided into three steps:Digestion of total cellular DNA with one or more restriction enzymes and ligation of restriction half-site specific adaptors to all restriction fragments.Selective amplification of some of these fragments with two PCR primers that have corresponding adaptor and restriction site specific sequences.Electrophoretic separation of amplicons on a gel matrix, followed by visualisation of the band pattern.A variation on AFLP is cDNA-AFLP, which is used to quantify differences in gene expression levels.Another variation on AFLP is TE Display, used to detect transposable element mobility.".
- Amplified_fragment_length_polymorphism thumbnail Electropherogram_trace.jpg?width=300.
- Amplified_fragment_length_polymorphism wikiPageExternalLink AFLP.
- Amplified_fragment_length_polymorphism wikiPageExternalLink index.php?mo=eukarya.
- Amplified_fragment_length_polymorphism wikiPageExternalLink user_seqs.
- Amplified_fragment_length_polymorphism wikiPageExternalLink genographer.
- Amplified_fragment_length_polymorphism wikiPageExternalLink rawgeno.
- Amplified_fragment_length_polymorphism wikiPageExternalLink import-curve-files-automated-sequencers.
- Amplified_fragment_length_polymorphism wikiPageExternalLink aflp.html.
- Amplified_fragment_length_polymorphism wikiPageExternalLink quantarsuite.php.
- Amplified_fragment_length_polymorphism wikiPageExternalLink index.php.
- Amplified_fragment_length_polymorphism wikiPageExternalLink www.neb.com.
- Amplified_fragment_length_polymorphism wikiPageExternalLink www.softgenetics.com.
- Amplified_fragment_length_polymorphism wikiPageExternalLink AFLPApplicationNote.pdf.
- Amplified_fragment_length_polymorphism wikiPageID "2203789".
- Amplified_fragment_length_polymorphism wikiPageRevisionID "590583033".
- Amplified_fragment_length_polymorphism hasPhotoCollection Amplified_fragment_length_polymorphism.
- Amplified_fragment_length_polymorphism subject Category:DNA.
- Amplified_fragment_length_polymorphism subject Category:DNA_profiling_techniques.
- Amplified_fragment_length_polymorphism subject Category:Molecular_biology.
- Amplified_fragment_length_polymorphism comment "AFLP-PCR or just AFLP is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by Keygene, AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments. A subset of the restriction fragments is then selected to be amplified.".
- Amplified_fragment_length_polymorphism label "AFLP".
- Amplified_fragment_length_polymorphism label "Amplification fragment length polymorphism".
- Amplified_fragment_length_polymorphism label "Amplified fragment length polymorphism".
- Amplified_fragment_length_polymorphism label "Amplified fragment length polymorphism".
- Amplified_fragment_length_polymorphism label "Polimorfismos en la longitud de fragmentos amplificados".
- Amplified_fragment_length_polymorphism label "Polymorphisme de longueur des fragments amplifiés".
- Amplified_fragment_length_polymorphism sameAs AFLP.
- Amplified_fragment_length_polymorphism sameAs Polimorfismos_en_la_longitud_de_fragmentos_amplificados.
- Amplified_fragment_length_polymorphism sameAs Polymorphisme_de_longueur_des_fragments_amplifiés.
- Amplified_fragment_length_polymorphism sameAs Polimorfisme_panjang_fragmen_teramplifikasi.
- Amplified_fragment_length_polymorphism sameAs Amplified_fragment_length_polymorphism.
- Amplified_fragment_length_polymorphism sameAs Amplification_fragment_length_polymorphism.
- Amplified_fragment_length_polymorphism sameAs m.06vpt7.
- Amplified_fragment_length_polymorphism sameAs Q292077.
- Amplified_fragment_length_polymorphism sameAs Q292077.
- Amplified_fragment_length_polymorphism wasDerivedFrom Amplified_fragment_length_polymorphism?oldid=590583033.
- Amplified_fragment_length_polymorphism depiction Electropherogram_trace.jpg.
- Amplified_fragment_length_polymorphism isPrimaryTopicOf Amplified_fragment_length_polymorphism.