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- BglII abstract "BglII (pronounced "bagel two") is a type II restriction endonuclease enzyme isolated from certain strains of Bacillus globigii. The principal function of restriction enzymes is the protection of the host genome against foreign DNA, but they may also have some involvement in recombination and transposition.Like most type II restriction enzymes, BglII consists of two identical subunits that form a homodimer around the DNA double helix. Each monomer is 223 amino acids and symmetrically bind both sides of the unique palindromic nucleotide sequence AGATCT, cleaving the scissile phosphodiester bond between the first Adenine and Guanine nucleotides on both strands of the DNA molecule, creating sticky ends with 5' end overhangs. Being a type II restriction enzyme, BglII does not require ATP (adenosine triphosphate) for its enzymatic function, but only requires association with a divalent metal cation, most likely Mg2+. Unlike other restriction enzymes of its class, BglII has been show to possess some unique structural characteristics, such as a β-sandwich subdomain, and appears to undergo a unique conformational change upon dimerization, but its overall structure and mechanism of catalysis remain consistent with other type II restriction enzymes. Restriction Endonuclease enzymes play a very important role in modern molecular cloning techniques. Because of their unique recognition/cut sites, restriction enzymes can be used to precisely and controlably cut DNA in specific locations. Once cut, scientist can then insert the desired DNA fragment possessing "sticky ends" into a circular DNA molecule, and ligate them together to create an engineered cloning vector.".
- BglII symbol "Endonuc-BglII".
- BglII thumbnail BglII_cut_sites.JPG?width=300.
- BglII wikiPageExternalLink index.html.
- BglII wikiPageExternalLink rebase.html.
- BglII wikiPageExternalLink result_flat.php4?ecno=3.1.21.4.
- BglII wikiPageExternalLink mmdbsrv.cgi?uid=48647.
- BglII wikiPageExternalLink viewer.fcgi?db=protein&id=20178143.
- BglII wikiPageExternalLink entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6173168&ordinalpos=1&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum.
- BglII wikiPageExternalLink entrez?db=protein&cmd=search&term=Q45488.
- BglII wikiPageExternalLink explore.do?structureId=1DFM.
- BglII wikiPageID "12678511".
- BglII wikiPageRevisionID "581290858".
- BglII caption "structure of restriction endonuclease bstyi bound to non-cognate dna".
- BglII hasPhotoCollection BglII.
- BglII interpro "IPR015278".
- BglII name "Restriction endonuclease BglII".
- BglII pfam "PF09195".
- BglII pfamClan "CL0236".
- BglII scop "1".
- BglII symbol "Endonuc-BglII".
- BglII subject Category:Bacterial_enzymes.
- BglII subject Category:Molecular_biology.
- BglII subject Category:Restriction_enzymes.
- BglII type Abstraction100002137.
- BglII type Activator114723079.
- BglII type BacterialEnzymes.
- BglII type Catalyst114723628.
- BglII type Chemical114806838.
- BglII type Compound114818238.
- BglII type Endonuclease114605415.
- BglII type Enzyme114732946.
- BglII type Macromolecule114944888.
- BglII type Material114580897.
- BglII type Matter100020827.
- BglII type Molecule114682133.
- BglII type Nuclease114963916.
- BglII type OrganicCompound114727670.
- BglII type Part113809207.
- BglII type PhysicalEntity100001930.
- BglII type Protein114728724.
- BglII type Relation100031921.
- BglII type RestrictionEndonuclease115005071.
- BglII type RestrictionEnzymes.
- BglII type Substance100019613.
- BglII type Thing100002452.
- BglII type Unit109465459.
- BglII type Biomolecule.
- BglII type Protein.
- BglII type BiologicalObject.
- BglII comment "BglII (pronounced "bagel two") is a type II restriction endonuclease enzyme isolated from certain strains of Bacillus globigii. The principal function of restriction enzymes is the protection of the host genome against foreign DNA, but they may also have some involvement in recombination and transposition.Like most type II restriction enzymes, BglII consists of two identical subunits that form a homodimer around the DNA double helix.".
- BglII label "BglII".
- BglII label "BglII".
- BglII label "BglII".
- BglII sameAs BglII.
- BglII sameAs BglII.
- BglII sameAs m.02x05w7.
- BglII sameAs Q4900470.
- BglII sameAs Q4900470.
- BglII sameAs BglII.
- BglII wasDerivedFrom BglII?oldid=581290858.
- BglII depiction BglII_cut_sites.JPG.
- BglII isPrimaryTopicOf BglII.