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- Bottom-up_proteomics abstract "Bottom-up proteomics is a common method to identify proteins and characterize their amino acid sequences and post-translational modifications by proteolytic digestion of proteins prior to analysis by mass spectrometry. The proteins may first be purified by a method such as gel electrophoresis resulting in one or a few proteins in each proteolytic digest. Alternatively, the crude protein extract is digested directly, followed by one or more dimensions of separation of the peptides by liquid chromatography coupled to mass spectrometry, a technique known as shotgun proteomics. By comparing the masses of the proteolytic peptides or their tandem mass spectra with those predicted from a sequence database or annotated peptide spectral in a peptide spectral library, peptides can be identified and multiple peptide identifications assembled into a protein identification.".
- Bottom-up_proteomics thumbnail Bottom-up_vs_top_down.svg?width=300.
- Bottom-up_proteomics wikiPageID "13688008".
- Bottom-up_proteomics wikiPageRevisionID "590805483".
- Bottom-up_proteomics hasPhotoCollection Bottom-up_proteomics.
- Bottom-up_proteomics subject Category:Bioinformatics.
- Bottom-up_proteomics subject Category:Mass_spectrometry.
- Bottom-up_proteomics subject Category:Proteomics.
- Bottom-up_proteomics comment "Bottom-up proteomics is a common method to identify proteins and characterize their amino acid sequences and post-translational modifications by proteolytic digestion of proteins prior to analysis by mass spectrometry. The proteins may first be purified by a method such as gel electrophoresis resulting in one or a few proteins in each proteolytic digest.".
- Bottom-up_proteomics label "Bottom-up proteomics".
- Bottom-up_proteomics sameAs m.03cf3zx.
- Bottom-up_proteomics sameAs Q4949102.
- Bottom-up_proteomics sameAs Q4949102.
- Bottom-up_proteomics wasDerivedFrom Bottom-up_proteomics?oldid=590805483.
- Bottom-up_proteomics depiction Bottom-up_vs_top_down.svg.
- Bottom-up_proteomics isPrimaryTopicOf Bottom-up_proteomics.