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- Bouin_solution abstract "Bouin solution is a compound fixative used in histology. It is composed of picric acid, acetic acid and formaldehyde in an aqueous solution. It is especially good for gastrointestinal tract biopsies because this fixative allows crisper and better nuclear staining than 10% neutral-buffered formalin. It is not a good fixative when tissue ultrastructure must be preserved for electron microscopy. However, it is a good fixative when tissue structure with a soft and delicate texture must be preserved. Formalin-fixed tissue is normally mordanted with Bouin solution for better staining results in the trichrome stains. The acetic acid in this fixative lyses red blood cells and dissolves small iron and calcium deposits in tissue. A variant in which the acetic acid is replaced with formic acid can be used for both fixation of tissue and decalcification.The effects of the three chemicals in Bouin solution balance each other. Formalin causes cytoplasm to become basophilic but this effect is balanced by the effect of the picric acid. This results in excellent nuclear and cytoplasmic H&E staining. The tissue hardening effect of formalin is balanced by the soft tissue fixation of picric acid. The tissue swelling effect of acetic acid is balanced by the tissue shrinking effect of picric acid.When using Bouin solution, several potential problems can arise. Due to the formalin in the solution, formalin pigment may be present when viewing tissue sections under the microscope. Wet tissue should be fixed in Bouin solution for less than 24 hours. Excess picric acid should be washed out of tissue using several alcohol and water solutions or staining quality may deteriorate over time. Wet tissue fixed in Bouin solution should be stored in an alcohol and water solution rather than Bouin solution. Since Bouin solution contains formaldehyde, picric acid and acetic acid, appropriate safety precautions for these substances should be taken and regulations followed. In particular, noting that picric acid can be explosive, sensitive to friction and shock when dry and in contact with some metals can form unstable metal picrates.Under the name "Bouin's fluid" this fixative is also widely used for marine invertebrates. It is prepared as follows: Picric acid, saturated aqueous solution - 75ml; Formalin, 40% aqueous solution - 25ml; Acetic acid, glacial - 5ml".
- Bouin_solution wikiPageExternalLink summary.cgi?cid=124013.
- Bouin_solution wikiPageID "31372356".
- Bouin_solution wikiPageRevisionID "556973802".
- Bouin_solution hasPhotoCollection Bouin_solution.
- Bouin_solution subject Category:Histology.
- Bouin_solution subject Category:Pathology.
- Bouin_solution comment "Bouin solution is a compound fixative used in histology. It is composed of picric acid, acetic acid and formaldehyde in an aqueous solution. It is especially good for gastrointestinal tract biopsies because this fixative allows crisper and better nuclear staining than 10% neutral-buffered formalin. It is not a good fixative when tissue ultrastructure must be preserved for electron microscopy.".
- Bouin_solution label "Bouin solution".
- Bouin_solution label "Liquide de Bouin".
- Bouin_solution label "محلول بوين".
- Bouin_solution sameAs Bouin-Lösung.
- Bouin_solution sameAs Liquide_de_Bouin.
- Bouin_solution sameAs m.0gk_zkx.
- Bouin_solution sameAs Q4949470.
- Bouin_solution sameAs Q4949470.
- Bouin_solution wasDerivedFrom Bouin_solution?oldid=556973802.
- Bouin_solution isPrimaryTopicOf Bouin_solution.