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- Chemically_defined_medium abstract "A chemically defined medium is a growth medium suitable for the in vitro cell culture of human or animal cells in which all of the chemical components are known. Standard cell culture media are commonly supplemented with animal serum (such as fetal bovine serum, FBS) as a source of nutrients and other ill-defined factors. The technical disadvantages to using serum include its undefined nature, batch-to-batch variability in composition, and the risk of contamination.There is a clear distinction between serum-free media and chemically defined media. Serum-free media may contain undefined animal-derived products such as serum albumin (purified from blood), hydrolysates, growth factors, hormones, carrier proteins, and attachment factors. These undefined animal-derived products will contain complex contaminants, such as the lipid content of albumin. In contrast, chemically defined media require that all of the components must be identified and have their exact concentrations known. Therefore a chemically defined medium must be entirely free of animal-derived components and cannot contain either fetal bovine serum, bovine serum albumin or human serum albumin. To achieve this chemically defined media is commonly supplemented with recombinant versions of albumin and growth factors, usually derived from rice or E. coli, or synthetic chemical such as the polymer polyvinyl alcohol which can reproduce some of the functions of BSA/HSA.The constituents of a chemically defined media include: a basal media (such as DMEM, F12, or RPMI 1640, containing amino acids, vitamins, inorganic salts, buffers, antioxidants and energy sources), which is supplemented with recombinant albumin, chemically defined lipids, recombinant insulin and/or zinc, recombinant transferrin or iron, selenium and a antioxidant thiol such as 2-mercaptoethanol or 1-thioglycerol.".
- Chemically_defined_medium wikiPageExternalLink products.
- Chemically_defined_medium wikiPageExternalLink 332-032442_SFMBrochure.pdf.
- Chemically_defined_medium wikiPageExternalLink PerspectivesWEB.pdf.
- Chemically_defined_medium wikiPageExternalLink page_13875.
- Chemically_defined_medium wikiPageID "31040311".
- Chemically_defined_medium wikiPageRevisionID "602649770".
- Chemically_defined_medium hasPhotoCollection Chemically_defined_medium.
- Chemically_defined_medium subject Category:Biotechnology.
- Chemically_defined_medium subject Category:Cell_biology.
- Chemically_defined_medium subject Category:Cell_culture.
- Chemically_defined_medium subject Category:Molecular_biology_techniques.
- Chemically_defined_medium type Ability105616246.
- Chemically_defined_medium type Abstraction100002137.
- Chemically_defined_medium type Cognition100023271.
- Chemically_defined_medium type Know-how105616786.
- Chemically_defined_medium type Method105660268.
- Chemically_defined_medium type MolecularBiologyTechniques.
- Chemically_defined_medium type PsychologicalFeature100023100.
- Chemically_defined_medium type Technique105665146.
- Chemically_defined_medium comment "A chemically defined medium is a growth medium suitable for the in vitro cell culture of human or animal cells in which all of the chemical components are known. Standard cell culture media are commonly supplemented with animal serum (such as fetal bovine serum, FBS) as a source of nutrients and other ill-defined factors.".
- Chemically_defined_medium label "Chemically defined medium".
- Chemically_defined_medium label "مستنبت محدد التركيب الكيميائي".
- Chemically_defined_medium sameAs m.0gg6gj6.
- Chemically_defined_medium sameAs Q5090513.
- Chemically_defined_medium sameAs Q5090513.
- Chemically_defined_medium sameAs Chemically_defined_medium.
- Chemically_defined_medium wasDerivedFrom Chemically_defined_medium?oldid=602649770.
- Chemically_defined_medium isPrimaryTopicOf Chemically_defined_medium.