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- Fluorescein_isothiocyanate abstract "Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. A succinimidyl-ester functional group attached to the fluorescein core, creating NHS-fluorescein, forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles. FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm. Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed.".
- Fluorescein_isothiocyanate thumbnail FITC-2D-skeletal.png?width=300.
- Fluorescein_isothiocyanate wikiPageID "2327550".
- Fluorescein_isothiocyanate wikiPageRevisionID "546068057".
- Fluorescein_isothiocyanate hasPhotoCollection Fluorescein_isothiocyanate.
- Fluorescein_isothiocyanate imagefile "FITC-2D-skeletal.png".
- Fluorescein_isothiocyanate othernames "FITC".
- Fluorescein_isothiocyanate verifiedrevid "443824480".
- Fluorescein_isothiocyanate subject Category:Fluorescent_dyes.
- Fluorescein_isothiocyanate subject Category:Fluorone_dyes.
- Fluorescein_isothiocyanate subject Category:Isothiocyanates.
- Fluorescein_isothiocyanate subject Category:Staining_dyes.
- Fluorescein_isothiocyanate type ChemicalCompound.
- Fluorescein_isothiocyanate type ChemicalSubstance.
- Fluorescein_isothiocyanate type ChemicalSubstanceType.
- Fluorescein_isothiocyanate type ChemicalObject.
- Fluorescein_isothiocyanate type Thing.
- Fluorescein_isothiocyanate comment "Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.".
- Fluorescein_isothiocyanate label "Fluorescein isothiocyanate".
- Fluorescein_isothiocyanate label "Isothiocyanate de fluorescéine".
- Fluorescein_isothiocyanate label "Isotiocianato de fluoresceína".
- Fluorescein_isothiocyanate label "Isotiocianato de fluoresceína".
- Fluorescein_isothiocyanate sameAs Isotiocianato_de_fluoresceína.
- Fluorescein_isothiocyanate sameAs Isothiocyanate_de_fluorescéine.
- Fluorescein_isothiocyanate sameAs Isotiocianato_de_fluoresceína.
- Fluorescein_isothiocyanate sameAs m.02p66q8.
- Fluorescein_isothiocyanate sameAs Q853845.
- Fluorescein_isothiocyanate sameAs Q853845.
- Fluorescein_isothiocyanate wasDerivedFrom Fluorescein_isothiocyanate?oldid=546068057.
- Fluorescein_isothiocyanate depiction FITC-2D-skeletal.png.
- Fluorescein_isothiocyanate isPrimaryTopicOf Fluorescein_isothiocyanate.