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- Gel_electrophoresis abstract "Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge. Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving. Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.".
- Gel_electrophoresis thumbnail Gel_electrophoresis_apparatus.JPG?width=300.
- Gel_electrophoresis wikiPageExternalLink index.html.
- Gel_electrophoresis wikiPageExternalLink virgel.html.
- Gel_electrophoresis wikiPageExternalLink index.php?title=Agarose_gel_electrophoresis&action=edit&redlink=1.
- Gel_electrophoresis wikiPageExternalLink gel.
- Gel_electrophoresis wikiPageExternalLink 417631.html.
- Gel_electrophoresis wikiPageExternalLink RDM1virtuallab.html.
- Gel_electrophoresis wikiPageExternalLink handbooks.
- Gel_electrophoresis wikiPageExternalLink ABSTRACT.
- Gel_electrophoresis wikiPageID "12582".
- Gel_electrophoresis wikiPageRevisionID "605459996".
- Gel_electrophoresis caption "Gel electrophoresis apparatus – An agarose gel is placed in this buffer-filled box and an electrical field is applied via the power supply to the rear. The negative terminal is at the far end , so DNA migrates toward the anode .".
- Gel_electrophoresis classification Electrophoresis.
- Gel_electrophoresis hasPhotoCollection Gel_electrophoresis.
- Gel_electrophoresis name "Gel electrophoresis".
- Gel_electrophoresis related Capillary_electrophoresis.
- Gel_electrophoresis related Polyacrylamide_gel_electrophoresis.
- Gel_electrophoresis related Temperature_gradient_gel_electrophoresis.
- Gel_electrophoresis related Two-dimensional_gel_electrophoresis.
- Gel_electrophoresis subject Category:Electrophoresis.
- Gel_electrophoresis subject Category:Laboratory_techniques.
- Gel_electrophoresis subject Category:Molecular_biology.
- Gel_electrophoresis subject Category:Polymerase_chain_reaction.
- Gel_electrophoresis subject Category:Protein_methods.
- Gel_electrophoresis type Ability105616246.
- Gel_electrophoresis type Abstraction100002137.
- Gel_electrophoresis type Act100030358.
- Gel_electrophoresis type Activity100407535.
- Gel_electrophoresis type Analysis100634276.
- Gel_electrophoresis type ChemicalAnalysis100646833.
- Gel_electrophoresis type Cognition100023271.
- Gel_electrophoresis type Event100029378.
- Gel_electrophoresis type Investigation100633864.
- Gel_electrophoresis type Know-how105616786.
- Gel_electrophoresis type LaboratoryTechniques.
- Gel_electrophoresis type Method105660268.
- Gel_electrophoresis type ProteinMethods.
- Gel_electrophoresis type PsychologicalFeature100023100.
- Gel_electrophoresis type Technique105665146.
- Gel_electrophoresis type Work100575741.
- Gel_electrophoresis type YagoPermanentlyLocatedEntity.
- Gel_electrophoresis comment "Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.".
- Gel_electrophoresis label "Electroforesis en gel".
- Gel_electrophoresis label "Eletroforese em gel".
- Gel_electrophoresis label "Gel di poliacrilammide".
- Gel_electrophoresis label "Gel electrophoresis".
- Gel_electrophoresis label "Gelelektroforese".
- Gel_electrophoresis label "Gelelektrophorese".
- Gel_electrophoresis label "Électrophorèse sur gel".
- Gel_electrophoresis label "Электрофорез в полиакриламидном геле".
- Gel_electrophoresis label "رحلان كهربي هلامي".
- Gel_electrophoresis label "二次元電気泳動".
- Gel_electrophoresis label "聚丙烯醯胺凝膠".
- Gel_electrophoresis sameAs Gelová_elektroforéza.
- Gel_electrophoresis sameAs Gelelektrophorese.
- Gel_electrophoresis sameAs Electroforesis_en_gel.
- Gel_electrophoresis sameAs Électrophorèse_sur_gel.
- Gel_electrophoresis sameAs Elektroforesis_gel.
- Gel_electrophoresis sameAs Gel_di_poliacrilammide.
- Gel_electrophoresis sameAs 二次元電気泳動.
- Gel_electrophoresis sameAs 겔_전기_영동법.
- Gel_electrophoresis sameAs Gelelektroforese.
- Gel_electrophoresis sameAs Eletroforese_em_gel.
- Gel_electrophoresis sameAs m.039k6.
- Gel_electrophoresis sameAs Q48255.
- Gel_electrophoresis sameAs Q48255.
- Gel_electrophoresis sameAs Gel_electrophoresis.
- Gel_electrophoresis wasDerivedFrom Gel_electrophoresis?oldid=605459996.
- Gel_electrophoresis depiction Gel_electrophoresis_apparatus.JPG.
- Gel_electrophoresis isPrimaryTopicOf Gel_electrophoresis.