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- HEPES abstract "HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent; one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES is like water in that its dissociation decreases as the temperature decreases. This makes HEPES a more effective buffering agent for maintaining enzyme structure and function at low temperatures. Lepe-Zuniga et al. reported a phototoxicity of HEPES when exposed to ambient light by the production of hydrogen peroxide, which is not a problem in bicarbonate-based cell culture buffers. It is therefore strongly advised to keep HEPES-containing solutions in darkness as much as possible. Fears that HEPES may serve as a nutrient source for aerobic bacteria have been shown to be unfounded.[citation needed]HEPES has the following characteristics: pKa (25 °C) = 7.5 Useful pH range = 6.8 to 8.2↑ ↑ ↑".
- HEPES iupacName "2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid".
- HEPES thumbnail HEPES.png?width=300.
- HEPES wikiPageExternalLink h3375.
- HEPES wikiPageExternalLink Suite.HsSigmaAdvancedSearch.formAction.
- HEPES wikiPageID "379954".
- HEPES wikiPageRevisionID "591632625".
- HEPES hasPhotoCollection HEPES.
- HEPES imagefile "HEPES.png".
- HEPES imagename "chemical structure of HEPES".
- HEPES imagesize "200".
- HEPES iupacname "2".
- HEPES name "HEPES".
- HEPES othernames "HEPES".
- HEPES verifiedrevid "448969371".
- HEPES watchedfields "changed".
- HEPES subject Category:Alcohols.
- HEPES subject Category:Amines.
- HEPES subject Category:Buffers.
- HEPES subject Category:Sulfonic_acids.
- HEPES type Abstraction100002137.
- HEPES type Acid114607521.
- HEPES type Agent114778436.
- HEPES type Alcohol107884567.
- HEPES type Alcohols.
- HEPES type AliphaticCompound114601294.
- HEPES type Amine114739004.
- HEPES type Amines.
- HEPES type Beverage107881800.
- HEPES type Buffer114785941.
- HEPES type Buffers.
- HEPES type CausalAgent100007347.
- HEPES type Chemical114806838.
- HEPES type Compound114818238.
- HEPES type Drug103247620.
- HEPES type DrugOfAbuse103248958.
- HEPES type Fluid114939900.
- HEPES type Food100021265.
- HEPES type Liquid114940386.
- HEPES type Material114580897.
- HEPES type Matter100020827.
- HEPES type MethaneSeries114951377.
- HEPES type OrganicCompound114727670.
- HEPES type Part113809207.
- HEPES type PhysicalEntity100001930.
- HEPES type Relation100031921.
- HEPES type Substance100019613.
- HEPES type Substance100020090.
- HEPES type SulfonicAcid114616508.
- HEPES type SulfonicAcids.
- HEPES type ChemicalCompound.
- HEPES type ChemicalSubstance.
- HEPES type ChemicalSubstanceType.
- HEPES type ChemicalObject.
- HEPES type Thing.
- HEPES comment "HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent; one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture.".
- HEPES label "HEPES".
- HEPES label "HEPES".
- HEPES label "HEPES".
- HEPES label "HEPES".
- HEPES label "HEPES".
- HEPES sameAs HEPES.
- HEPES sameAs HEPES.
- HEPES sameAs HEPES.
- HEPES sameAs HEPES.
- HEPES sameAs m.021gbn.
- HEPES sameAs Q418359.
- HEPES sameAs Q418359.
- HEPES sameAs HEPES.
- HEPES wasDerivedFrom HEPES?oldid=591632625.
- HEPES depiction HEPES.png.
- HEPES isPrimaryTopicOf HEPES.
- HEPES name "HEPES".