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- In_situ_hybridization abstract "In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA or RNA strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue (in situ), or, if the tissue is small enough (e.g. plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells and in circulating tumor cells (CTCs). This is distinct from immunohistochemistry, which usually localizes proteins in tissue sections. In situ hybridization is a powerful technique for identifying specific mRNA species within individual cells in tissue sections, providing insights into physiological processes and disease pathogenesis. However, in situ hybridization requires that many steps be taken with precise optimization for each tissue examined and for each probe used. In order to preserve the target mRNA within tissues, it is often required that crosslinking fixatives (such as formalin) be used.In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues, a crucial step for understanding the organization, regulation and function of genes. The key techniques currently in use include: in situ hybridization to mRNA with oligonucleotide and RNA probes (both radio labelled and hapten labelled); analysis with light and electron microscopes; whole mount in situ hybridization; double detection of RNAs and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences. DNA ISH can be used to determine the structure of chromosomes. Fluorescent DNA ISH (FISH) can, for example, be used in medical diagnostics to assess chromosomal integrity. RNA ISH (RNA in situ hybridization) is used to measure and localize RNAs (mRNAs, lncRNAs and miRNAs) within tissue sections, cells, whole mounts, and circulating tumor cells (CTCs). In situ hybridization was invented by Joseph G. Gall.".
- In_situ_hybridization thumbnail RNA_in_situ_hybridization_in_FFPE_samples.jpg?width=300.
- In_situ_hybridization wikiPageExternalLink TF2005.html.
- In_situ_hybridization wikiPageExternalLink ISHH.html.
- In_situ_hybridization wikiPageExternalLink S0896-6273(12)00286-3.
- In_situ_hybridization wikiPageExternalLink pdb.prot4519.
- In_situ_hybridization wikiPageExternalLink pdb.prot4730.
- In_situ_hybridization wikiPageExternalLink pdb.prot4944.
- In_situ_hybridization wikiPageExternalLink ee.
- In_situ_hybridization wikiPageExternalLink aboutGXD.shtml.
- In_situ_hybridization wikiPageExternalLink 22763454.
- In_situ_hybridization wikiPageExternalLink index.php?id=products_viewrnaoverview.
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- In_situ_hybridization wikiPageID "2313597".
- In_situ_hybridization wikiPageRevisionID "596707804".
- In_situ_hybridization hasPhotoCollection In_situ_hybridization.
- In_situ_hybridization subject Category:Biological_techniques_and_tools.
- In_situ_hybridization subject Category:Laboratory_techniques.
- In_situ_hybridization type Ability105616246.
- In_situ_hybridization type Abstraction100002137.
- In_situ_hybridization type BiologicalTechniquesAndTools.
- In_situ_hybridization type Cognition100023271.
- In_situ_hybridization type Know-how105616786.
- In_situ_hybridization type LaboratoryTechniques.
- In_situ_hybridization type Method105660268.
- In_situ_hybridization type PsychologicalFeature100023100.
- In_situ_hybridization type Technique105665146.
- In_situ_hybridization comment "In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA or RNA strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue (in situ), or, if the tissue is small enough (e.g. plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells and in circulating tumor cells (CTCs). This is distinct from immunohistochemistry, which usually localizes proteins in tissue sections.".
- In_situ_hybridization label "Hibridación in situ".
- In_situ_hybridization label "Hybridation in situ".
- In_situ_hybridization label "In situ hybridization".
- In_situ_hybridization label "In situ ハイブリダイゼーション".
- In_situ_hybridization label "In-situ-Hybridisierung".
- In_situ_hybridization label "تهجين موضعي".
- In_situ_hybridization sameAs In-situ-Hybridisierung.
- In_situ_hybridization sameAs Hibridación_in_situ.
- In_situ_hybridization sameAs Hybridation_in_situ.
- In_situ_hybridization sameAs In_situ_ハイブリダイゼーション.
- In_situ_hybridization sameAs m.0732t1.
- In_situ_hybridization sameAs Q2304142.
- In_situ_hybridization sameAs Q2304142.
- In_situ_hybridization sameAs In_situ_hybridization.
- In_situ_hybridization wasDerivedFrom In_situ_hybridization?oldid=596707804.
- In_situ_hybridization depiction RNA_in_situ_hybridization_in_FFPE_samples.jpg.
- In_situ_hybridization isPrimaryTopicOf In_situ_hybridization.
