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- Isotope-coded_affinity_tag abstract "Isotope-coded affinity tags (ICATs) are a gel-free method for quantitative proteomics that relies on chemical labeling reagents. These chemical probes consist of three general elements: a reactive group capable of labeling a defined amino acid side chain (e.g., iodoacetamide to modify cysteine residues), an isotopically coded linker, and a tag (e.g., biotin) for the affinity isolation of labeled proteins/peptides. For the quantitative comparison of two proteomes, one sample is labeled with the isotopically light (d0) probe and the other with the isotopically heavy (d8) version. To minimize error, both samples are then combined, digested with a protease (i.e., trypsin), and subjected to avidin affinity chromatography to isolate peptides labeled with isotope-coded tagging reagents. These peptides are then analyzed by liquid chromatography-mass spectrometry (LC-MS). The ratios of signal intensities of differentially mass-tagged peptide pairs are quantified to determine the relative levels of proteins in the two samples.The original tags were developed using deuterium, but later the same group redesigned the tags using 13C instead to circumvent issues of peak separation during LC due to the deuterium interacting with the stationary phase of the column.".
- Isotope-coded_affinity_tag wikiPageExternalLink quantitative-proteomics.
- Isotope-coded_affinity_tag wikiPageID "19973412".
- Isotope-coded_affinity_tag wikiPageRevisionID "591865578".
- Isotope-coded_affinity_tag hasPhotoCollection Isotope-coded_affinity_tag.
- Isotope-coded_affinity_tag subject Category:Proteomics.
- Isotope-coded_affinity_tag comment "Isotope-coded affinity tags (ICATs) are a gel-free method for quantitative proteomics that relies on chemical labeling reagents. These chemical probes consist of three general elements: a reactive group capable of labeling a defined amino acid side chain (e.g., iodoacetamide to modify cysteine residues), an isotopically coded linker, and a tag (e.g., biotin) for the affinity isolation of labeled proteins/peptides.".
- Isotope-coded_affinity_tag label "ICAT".
- Isotope-coded_affinity_tag label "Isotope-coded affinity tag".
- Isotope-coded_affinity_tag sameAs ICAT.
- Isotope-coded_affinity_tag sameAs m.04yh9qc.
- Isotope-coded_affinity_tag sameAs Q6086445.
- Isotope-coded_affinity_tag sameAs Q6086445.
- Isotope-coded_affinity_tag wasDerivedFrom Isotope-coded_affinity_tag?oldid=591865578.
- Isotope-coded_affinity_tag isPrimaryTopicOf Isotope-coded_affinity_tag.