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- Most_probable_number abstract "The most probable number method, otherwise known as the method of Poisson zeroes, is a method of getting quantitative data on concentrations of discrete items from positive/negative (incidence) data.There are many discrete entities that are easily detected but difficult to count. Any sort of amplification reaction or catalysis reaction obliterates easy quantification but allows presence to be detected very sensitively. Common examples include microorganism growth, enzyme action, or catalytic chemistry. The MPN method involves taking the original solution or sample, and subdividing it by orders of magnitude (frequently 10× or 2×), and assessing presence/absence in multiple subdivisions. The degree of dilution at which absence begins to appear indicates that the items have been diluted so much that there are many subsamples in which none appear. A suite of replicates at any given concentration allow finer resolution, to use the number of positive and negative samples to estimate the original concentration within the appropriate order of magnitude.In microbiology, the cultures are incubated and assessed by eye, bypassing tedious colony counting or expensive and tedious microscopic counts.In molecular biology, a common application involves DNA templates diluted into polymerase chain reaction (PCR) reactions. Reactions only proceed when a template is present, allowing for a form of quantitative PCR, to assess the original concentration of template molecules. Another application involves diluting enzyme stocks into solution containing a chromogenic substrate, or diluting antigens into solutions for ELISA (Enzyme-Linked ImmunoSorbent Assay) or some other antibody cascade detection reaction, to measure the original concentration of the enzyme or antigen.The major weakness of MPN methods is the need for large numbers of replicates at the appropriate dilution to narrow the confidence intervals. However, it is a very important method for counts when the appropriate order of magnitude is unknown a priori and sampling is necessarily destructive.".
- Most_probable_number wikiPageExternalLink ucm109656.htm.
- Most_probable_number wikiPageExternalLink index.html.
- Most_probable_number wikiPageExternalLink 102dil3.html.
- Most_probable_number wikiPageExternalLink 102dil3a.html.
- Most_probable_number wikiPageID "15151281".
- Most_probable_number wikiPageRevisionID "601974890".
- Most_probable_number hasPhotoCollection Most_probable_number.
- Most_probable_number subject Category:Biostatistics.
- Most_probable_number subject Category:Laboratory_techniques.
- Most_probable_number subject Category:Pharmacology.
- Most_probable_number subject Category:Quantitative_research.
- Most_probable_number type Ability105616246.
- Most_probable_number type Abstraction100002137.
- Most_probable_number type Cognition100023271.
- Most_probable_number type Know-how105616786.
- Most_probable_number type LaboratoryTechniques.
- Most_probable_number type Method105660268.
- Most_probable_number type PsychologicalFeature100023100.
- Most_probable_number type Technique105665146.
- Most_probable_number comment "The most probable number method, otherwise known as the method of Poisson zeroes, is a method of getting quantitative data on concentrations of discrete items from positive/negative (incidence) data.There are many discrete entities that are easily detected but difficult to count. Any sort of amplification reaction or catalysis reaction obliterates easy quantification but allows presence to be detected very sensitively.".
- Most_probable_number label "Most Probable Number".
- Most_probable_number label "Most probable number".
- Most_probable_number label "Número más probable".
- Most_probable_number label "Titerverfahren".
- Most_probable_number sameAs Titerverfahren.
- Most_probable_number sameAs Número_más_probable.
- Most_probable_number sameAs Most_Probable_Number.
- Most_probable_number sameAs m.03hk2tn.
- Most_probable_number sameAs Q3866144.
- Most_probable_number sameAs Q3866144.
- Most_probable_number sameAs Most_probable_number.
- Most_probable_number wasDerivedFrom Most_probable_number?oldid=601974890.
- Most_probable_number isPrimaryTopicOf Most_probable_number.