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- Nile_red abstract "Nile red (also known as Nile blue oxazone) is a lipophilic stain. It is produced by boiling a solution of Nile blue with sulfuric acid. As can be seen from the structural formulae, this process replaces an iminium group with a carbonyl group. Nile red stains intracellular lipid droplets red. In most polar solvents Nile Red will not fluoresce, however when in a lipid-rich environment can be intensely fluorescent, with varying colours from deep red to strong yellow-gold emission. Whilst it generally excites at 485 nm, and emits at 525 nm (552/636 nm in methanol), the fluorescence of the dye is heavily dependent on the solvent used, and in some cases does not fluoresce at all.Since the reaction to generate Nile red does not usually completely exhaust the supply of Nile blue, additional separation steps are required if pure Nile red is needed.Nile red has applications in cell biology, where it can be used as a membrane dye which can be readily visualised using an epifluorescence microscope with excitation and emission wavelengths usually shared with RFP.".
- Nile_red iupacName "9-diethylamino-5-benzo[α]phenoxazinone".
- Nile_red thumbnail Nile_red.svg?width=300.
- Nile_red wikiPageID "1574353".
- Nile_red wikiPageRevisionID "587282322".
- Nile_red hasPhotoCollection Nile_red.
- Nile_red imagefile "Nile red.svg".
- Nile_red imagefile "Nile-red-3D-balls.png".
- Nile_red imagesize "200".
- Nile_red iupacname "9".
- Nile_red othernames "Nile red, Nile blue oxazone".
- Nile_red verifiedfields "changed".
- Nile_red verifiedrevid "476992673".
- Nile_red subject Category:Fluorescent_dyes.
- Nile_red subject Category:Naphthobenzoxazines.
- Nile_red subject Category:Oxazin_dyes.
- Nile_red subject Category:Vital_stains.
- Nile_red type AbsorptionIndicator114598383.
- Nile_red type Abstraction100002137.
- Nile_red type Appearance104673965.
- Nile_red type Attribute100024264.
- Nile_red type ColoringMaterial114984973.
- Nile_red type Dye114985383.
- Nile_red type Fluorescein114987695.
- Nile_red type FluorescentDyes.
- Nile_red type Indicator114917208.
- Nile_red type Material114580897.
- Nile_red type Matter100020827.
- Nile_red type OxazinDyes.
- Nile_red type Part113809207.
- Nile_red type PhysicalEntity100001930.
- Nile_red type Quality104723816.
- Nile_red type Relation100031921.
- Nile_red type Stain104695176.
- Nile_red type Substance100019613.
- Nile_red type VitalStains.
- Nile_red type ChemicalCompound.
- Nile_red type ChemicalSubstance.
- Nile_red type ChemicalSubstanceType.
- Nile_red type ChemicalObject.
- Nile_red type Thing.
- Nile_red comment "Nile red (also known as Nile blue oxazone) is a lipophilic stain. It is produced by boiling a solution of Nile blue with sulfuric acid. As can be seen from the structural formulae, this process replaces an iminium group with a carbonyl group. Nile red stains intracellular lipid droplets red. In most polar solvents Nile Red will not fluoresce, however when in a lipid-rich environment can be intensely fluorescent, with varying colours from deep red to strong yellow-gold emission.".
- Nile_red label "Nile red".
- Nile_red label "Nilrot".
- Nile_red sameAs Nilrot.
- Nile_red sameAs m.02p4jg2.
- Nile_red sameAs Q910572.
- Nile_red sameAs Q910572.
- Nile_red sameAs Nile_red.
- Nile_red wasDerivedFrom Nile_red?oldid=587282322.
- Nile_red depiction Nile_red.svg.
- Nile_red isPrimaryTopicOf Nile_red.