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- Photo-reactive_amino_acid_analog abstract "Photo-reactive amino acid analogs are artificial analogs of natural amino acids that can be used for crosslinking of protein complexes. Photo-reactive amino acid analogs may be incorporated into proteins and peptides in vivo or in vitro. Photo-reactive amino acid analogs in common use are photoreactive diazirine analogs to leucine and methionine, and p-benzoylphenylalanine. Upon exposure to ultraviolet light, they are activated and covalently bind to interacting proteins that are within a few angstroms of the photo-reactive amino acid analog.L-Photo-Leucine and L-Photo-Methionine are analogs of the naturally occurring L-Leucine and L-Methionine amino acids that are endogenously incorporated into the primary sequence of proteins during synthesis using the normal translation machinery. They are then ultraviolet light (UV)-activated to covalently crosslink proteins within protein–protein interaction domains in their native in-vivo environment. The method enables the determination and characterization of both stable and transient protein interactions in cells without the addition of chemical crosslinkers and associated solvents that can adversely affect the cell biology being studied in the experiment. When used in combination with limiting media that is devoid of leucine and methionine, the photo-activatable derivatives are treated like naturally occurring amino acids by the cellular protein synthesis machinery. As a result, they can be substituted for leucine or methionine in the primary structure of proteins. Photo-leucine and photo-methionine derivatives contain diazirine rings that are activated when exposed to UV light to become reactive intermediates that form covalent bonds with nearby protein side chains and backbones. Naturally interacting proteins within the cell can be instantly trapped by photoactivation of the diazirine-containing proteins in the cultured cells. Crosslinked protein complexes can be detected by decreased mobility on SDS-PAGE followed by Western blotting, size exclusion chromatography, sucrose density gradient sedimentation or mass spectrometry.".
- Photo-reactive_amino_acid_analog wikiPageExternalLink chitem.aspx?aid=2427.
- Photo-reactive_amino_acid_analog wikiPageID "14136041".
- Photo-reactive_amino_acid_analog wikiPageRevisionID "594675257".
- Photo-reactive_amino_acid_analog hasPhotoCollection Photo-reactive_amino_acid_analog.
- Photo-reactive_amino_acid_analog subject Category:Biochemistry_methods.
- Photo-reactive_amino_acid_analog subject Category:Biotechnology.
- Photo-reactive_amino_acid_analog subject Category:Protein–protein_interaction_assays.
- Photo-reactive_amino_acid_analog subject Category:Proteomics.
- Photo-reactive_amino_acid_analog type Ability105616246.
- Photo-reactive_amino_acid_analog type Abstraction100002137.
- Photo-reactive_amino_acid_analog type Appraisal105733583.
- Photo-reactive_amino_acid_analog type Assay105738625.
- Photo-reactive_amino_acid_analog type BasicCognitiveProcess105701944.
- Photo-reactive_amino_acid_analog type BiochemistryMethods.
- Photo-reactive_amino_acid_analog type Classification105732756.
- Photo-reactive_amino_acid_analog type Cognition100023271.
- Photo-reactive_amino_acid_analog type Know-how105616786.
- Photo-reactive_amino_acid_analog type Method105660268.
- Photo-reactive_amino_acid_analog type Process105701363.
- Photo-reactive_amino_acid_analog type Protein-proteinInteractionAssays.
- Photo-reactive_amino_acid_analog type PsychologicalFeature100023100.
- Photo-reactive_amino_acid_analog comment "Photo-reactive amino acid analogs are artificial analogs of natural amino acids that can be used for crosslinking of protein complexes. Photo-reactive amino acid analogs may be incorporated into proteins and peptides in vivo or in vitro. Photo-reactive amino acid analogs in common use are photoreactive diazirine analogs to leucine and methionine, and p-benzoylphenylalanine.".
- Photo-reactive_amino_acid_analog label "Photo-reactive amino acid analog".
- Photo-reactive_amino_acid_analog label "نظائر الأحماض الأمينية المفاعلة للضوء".
- Photo-reactive_amino_acid_analog sameAs m.03cvpnc.
- Photo-reactive_amino_acid_analog sameAs Q7187642.
- Photo-reactive_amino_acid_analog sameAs Q7187642.
- Photo-reactive_amino_acid_analog sameAs Photo-reactive_amino_acid_analog.
- Photo-reactive_amino_acid_analog wasDerivedFrom Photo-reactive_amino_acid_analog?oldid=594675257.
- Photo-reactive_amino_acid_analog isPrimaryTopicOf Photo-reactive_amino_acid_analog.