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- Quantitative_phase-contrast_microscopy abstract "Quantitative phase contrast microscopy is the collective name for a group of microscopy methods that quantify the phase shift that occurs when light waves pass through a more optically dense object.Translucent objects, like a living human cell, absorb and scatter small amounts of light.This makes translucent objects difficult to observe in ordinary light microscopes.Such objects do, however, induce a phase shift that can be observed using either a phase contrast microscope.Conventional phase contrast microscopy and related methods, such as differential interference contrast microscopy,visualize phase shifts by transforming phase shift gradients into intensity variations.These intensity variations are mixed with other intensity variations, making it difficult to extract quantitative information.Quantitative phase contrast methods are distinguished from other phase contrast methods in thatthey create a second so-called phase shift image or phase image, independent of the intensity (bright field) image.Phase unwrapping methods are generally applied to the phase shift image to give absolute phase shiftvalues in each pixel, as exemplified by Figure 1.The principal methods for measuring and visualizing phase shifts include ptychography and various typesof holographic microscopy methods such as digital holographic microscopy,holographic interference microscopy and digital in-line holographic microscopy.Common to these methods is that an interference pattern (hologram) is recorded by a digital image sensor.From the recorded interference pattern, the intensity and the phase shift image is numerically created by a computer algorithm.Conventional phase contrast microscopy is primarily used to observed unstained living cells.Contrary to conventional phase contrast images, phase shift images of living cells are suitable to be processed by image analysis software.This has led to the development of non-invasive live cell imaging and automated cell culture analysis systems based on quantitative phase contrast microscopy.".
- Quantitative_phase-contrast_microscopy thumbnail HoloMonitor_M4_with_IBIDI_slide.png?width=300.
- Quantitative_phase-contrast_microscopy wikiPageExternalLink 43451.
- Quantitative_phase-contrast_microscopy wikiPageExternalLink www.lynceetec.com.
- Quantitative_phase-contrast_microscopy wikiPageExternalLink .UIeXM2fmGSo.
- Quantitative_phase-contrast_microscopy wikiPageExternalLink www.ovizio.com.
- Quantitative_phase-contrast_microscopy wikiPageExternalLink www.phasefocus.com.
- Quantitative_phase-contrast_microscopy wikiPageExternalLink www.phiab.se.
- Quantitative_phase-contrast_microscopy wikiPageID "37417891".
- Quantitative_phase-contrast_microscopy wikiPageRevisionID "603832270".
- Quantitative_phase-contrast_microscopy acronym "QPCM".
- Quantitative_phase-contrast_microscopy caption "A quantitative phase contrast microscope imaging cultured cells in a slide based cell culture vessel.".
- Quantitative_phase-contrast_microscopy manufacturer "LynceeTec, pionner in DHMPhase Holographic Imaging, Phase Focus Ltd Ovizio Imaging Systems and others".
- Quantitative_phase-contrast_microscopy name "Quantitative phase contrast microscope".
- Quantitative_phase-contrast_microscopy otherNames "Phase microscope, Quantitative phase microscopy".
- Quantitative_phase-contrast_microscopy related Differential_interference_contrast_microscopy.
- Quantitative_phase-contrast_microscopy related Hoffman_modulation_contrast.
- Quantitative_phase-contrast_microscopy related Phase_contrast_microscopy.
- Quantitative_phase-contrast_microscopy related Ptychography.
- Quantitative_phase-contrast_microscopy uses "Microscopic observation and quantification of unstained biological material".
- Quantitative_phase-contrast_microscopy subject Category:Cell_imaging.
- Quantitative_phase-contrast_microscopy subject Category:Laboratory_techniques.
- Quantitative_phase-contrast_microscopy subject Category:Microbiology_techniques.
- Quantitative_phase-contrast_microscopy subject Category:Microscopes.
- Quantitative_phase-contrast_microscopy subject Category:Microscopy.
- Quantitative_phase-contrast_microscopy comment "Quantitative phase contrast microscopy is the collective name for a group of microscopy methods that quantify the phase shift that occurs when light waves pass through a more optically dense object.Translucent objects, like a living human cell, absorb and scatter small amounts of light.This makes translucent objects difficult to observe in ordinary light microscopes.Such objects do, however, induce a phase shift that can be observed using either a phase contrast microscope.Conventional phase contrast microscopy and related methods, such as differential interference contrast microscopy,visualize phase shifts by transforming phase shift gradients into intensity variations.These intensity variations are mixed with other intensity variations, making it difficult to extract quantitative information.Quantitative phase contrast methods are distinguished from other phase contrast methods in thatthey create a second so-called phase shift image or phase image, independent of the intensity (bright field) image.Phase unwrapping methods are generally applied to the phase shift image to give absolute phase shiftvalues in each pixel, as exemplified by Figure 1.The principal methods for measuring and visualizing phase shifts include ptychography and various typesof holographic microscopy methods such as digital holographic microscopy,holographic interference microscopy and digital in-line holographic microscopy.Common to these methods is that an interference pattern (hologram) is recorded by a digital image sensor.From the recorded interference pattern, the intensity and the phase shift image is numerically created by a computer algorithm.Conventional phase contrast microscopy is primarily used to observed unstained living cells.Contrary to conventional phase contrast images, phase shift images of living cells are suitable to be processed by image analysis software.This has led to the development of non-invasive live cell imaging and automated cell culture analysis systems based on quantitative phase contrast microscopy.".
- Quantitative_phase-contrast_microscopy label "Quantitative phase-contrast microscopy".
- Quantitative_phase-contrast_microscopy sameAs m.0nb8b68.
- Quantitative_phase-contrast_microscopy sameAs Q7268937.
- Quantitative_phase-contrast_microscopy sameAs Q7268937.
- Quantitative_phase-contrast_microscopy wasDerivedFrom Quantitative_phase-contrast_microscopy?oldid=603832270.
- Quantitative_phase-contrast_microscopy depiction HoloMonitor_M4_with_IBIDI_slide.png.
- Quantitative_phase-contrast_microscopy isPrimaryTopicOf Quantitative_phase-contrast_microscopy.