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- Ribosomal_Intergenic_Spacer_analysis abstract "Ribosomal RNA (rRNA) intergenic spacer analysis (RISA) is a method of microbial community analysis that provides a means of comparing differing environments or treatment impacts without the bias imposed by culture- dependent approaches. This type of analysis is often referred to as community fingerprinting. RISA involves PCR amplification of a region of the rRNA gene operon between the small (16S) and large (23S) subunits called the intergenic spacer region ISR. By using oligonucleotide primers targeted to conserved regions in the 16S and 23S genes, RISA fragments can be generated from most of the dominant bacteria in an environmental sample. While the majority of the rRNA operon serves a structural function, portions of the 16S-23S intergenic region can encode tRNAs depending on the bacterial species. However the taxonomic value of the ISR lies in the significant heterogeneity in both length and nucleotide sequence. In RISA, we attempt to exploit the length heterogeneity of the ISR, which has been shown to range between 150 and 1500 bp with the majority of the ISR lengths being between 150 and 500 bp.The resulting PCR product will be a mixture of fragments contributed by several dominant community members. This product is electrophoresed in a polyacrylamide gel, and the DNA is visualized following staining. The result is a complex banding pattern that provides a community-specific profile, with each DNA band corresponding to a bacterial population on the original assemblage.".
- Ribosomal_Intergenic_Spacer_analysis wikiPageID "25271931".
- Ribosomal_Intergenic_Spacer_analysis wikiPageRevisionID "551897945".
- Ribosomal_Intergenic_Spacer_analysis hasPhotoCollection Ribosomal_Intergenic_Spacer_analysis.
- Ribosomal_Intergenic_Spacer_analysis subject Category:Biotechnology.
- Ribosomal_Intergenic_Spacer_analysis subject Category:DNA_profiling_techniques.
- Ribosomal_Intergenic_Spacer_analysis subject Category:Laboratory_techniques.
- Ribosomal_Intergenic_Spacer_analysis subject Category:Molecular_biology.
- Ribosomal_Intergenic_Spacer_analysis subject Category:Molecular_biology_techniques.
- Ribosomal_Intergenic_Spacer_analysis type Ability105616246.
- Ribosomal_Intergenic_Spacer_analysis type Abstraction100002137.
- Ribosomal_Intergenic_Spacer_analysis type Cognition100023271.
- Ribosomal_Intergenic_Spacer_analysis type Know-how105616786.
- Ribosomal_Intergenic_Spacer_analysis type LaboratoryTechniques.
- Ribosomal_Intergenic_Spacer_analysis type Method105660268.
- Ribosomal_Intergenic_Spacer_analysis type MolecularBiologyTechniques.
- Ribosomal_Intergenic_Spacer_analysis type PsychologicalFeature100023100.
- Ribosomal_Intergenic_Spacer_analysis type Technique105665146.
- Ribosomal_Intergenic_Spacer_analysis comment "Ribosomal RNA (rRNA) intergenic spacer analysis (RISA) is a method of microbial community analysis that provides a means of comparing differing environments or treatment impacts without the bias imposed by culture- dependent approaches. This type of analysis is often referred to as community fingerprinting. RISA involves PCR amplification of a region of the rRNA gene operon between the small (16S) and large (23S) subunits called the intergenic spacer region ISR.".
- Ribosomal_Intergenic_Spacer_analysis label "Ribosomal Intergenic Spacer analysis".
- Ribosomal_Intergenic_Spacer_analysis label "تحليل المفساح بين الجينات للرَّنا الرِّيباسِيّ".
- Ribosomal_Intergenic_Spacer_analysis sameAs m.09g87tz.
- Ribosomal_Intergenic_Spacer_analysis sameAs Q7322402.
- Ribosomal_Intergenic_Spacer_analysis sameAs Q7322402.
- Ribosomal_Intergenic_Spacer_analysis sameAs Ribosomal_Intergenic_Spacer_analysis.
- Ribosomal_Intergenic_Spacer_analysis wasDerivedFrom Ribosomal_Intergenic_Spacer_analysis?oldid=551897945.
- Ribosomal_Intergenic_Spacer_analysis isPrimaryTopicOf Ribosomal_Intergenic_Spacer_analysis.