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- Synapto-pHluorin abstract "Synapto-pHluorin is a genetically encoded optical indicator of vesicle release and recycling. It is used in neuroscience to study transmitter release. It consists of a pH-sensitive form of green fluorescent protein (GFP) fused to the luminal side of a vesicle-associated membrane protein (VAMP). At the acidic pH inside transmitter vesicles, synapto-pHluorin is non-fluorescent. When vesicles get released, synapto-pHluorin is exposed to the neutral extracellular space and the presynaptic terminal becomes brightly fluorescent. Following endocytosis, vesicles become re-acidified and the cycle can start again. Chemical alkalinization of all vesicles is often used for normalization of the synapto-pHluorin signals. In the following video a typical Synapto-pHluorin experiment is shown (VGluT1-pHluorin in this case).REDIRECT [1]".
- Synapto-pHluorin wikiPageExternalLink watch?v=RrJE4gNI_ec.
- Synapto-pHluorin wikiPageID "21377466".
- Synapto-pHluorin wikiPageRevisionID "558827728".
- Synapto-pHluorin hasPhotoCollection Synapto-pHluorin.
- Synapto-pHluorin subject Category:Cell_imaging.
- Synapto-pHluorin subject Category:Fluorescent_dyes.
- Synapto-pHluorin subject Category:Protein_methods.
- Synapto-pHluorin subject Category:Recombinant_proteins.
- Synapto-pHluorin type Ability105616246.
- Synapto-pHluorin type AbsorptionIndicator114598383.
- Synapto-pHluorin type Abstraction100002137.
- Synapto-pHluorin type Chemical114806838.
- Synapto-pHluorin type Cognition100023271.
- Synapto-pHluorin type ColoringMaterial114984973.
- Synapto-pHluorin type Compound114818238.
- Synapto-pHluorin type Dye114985383.
- Synapto-pHluorin type Fluorescein114987695.
- Synapto-pHluorin type FluorescentDyes.
- Synapto-pHluorin type Indicator114917208.
- Synapto-pHluorin type Know-how105616786.
- Synapto-pHluorin type Macromolecule114944888.
- Synapto-pHluorin type Material114580897.
- Synapto-pHluorin type Matter100020827.
- Synapto-pHluorin type Method105660268.
- Synapto-pHluorin type Molecule114682133.
- Synapto-pHluorin type OrganicCompound114727670.
- Synapto-pHluorin type Part113809207.
- Synapto-pHluorin type PhysicalEntity100001930.
- Synapto-pHluorin type Protein114728724.
- Synapto-pHluorin type ProteinMethods.
- Synapto-pHluorin type PsychologicalFeature100023100.
- Synapto-pHluorin type RecombinantProtein114729633.
- Synapto-pHluorin type RecombinantProteins.
- Synapto-pHluorin type Relation100031921.
- Synapto-pHluorin type Substance100019613.
- Synapto-pHluorin type Thing100002452.
- Synapto-pHluorin type Unit109465459.
- Synapto-pHluorin comment "Synapto-pHluorin is a genetically encoded optical indicator of vesicle release and recycling. It is used in neuroscience to study transmitter release. It consists of a pH-sensitive form of green fluorescent protein (GFP) fused to the luminal side of a vesicle-associated membrane protein (VAMP). At the acidic pH inside transmitter vesicles, synapto-pHluorin is non-fluorescent.".
- Synapto-pHluorin label "Synapto-pHluorin".
- Synapto-pHluorin sameAs m.05f4ssb.
- Synapto-pHluorin sameAs Q7662044.
- Synapto-pHluorin sameAs Q7662044.
- Synapto-pHluorin sameAs Synapto-pHluorin.
- Synapto-pHluorin wasDerivedFrom Synapto-pHluorin?oldid=558827728.
- Synapto-pHluorin isPrimaryTopicOf Synapto-pHluorin.