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- Texas_Red abstract "Texas Red or sulforhodamine 101 acid chloride is a red fluorescent dye, used in histology for staining cell specimens, for sorting cells with fluorescent-activated cell sorting machines, in fluorescence microscopy applications, and in immunohistochemistry.Texas Red fluoresces at about 615 nm, and the peak of its absorption spectrum is at 589 nm. The powder is dark purple. Solutions can be excited by a dye laser tuned to 595-605 nm, or less efficiently a krypton laser at 567 nm. The absorption extinction coefficient at 596 nm is about 85,000 M−1cm−1. The compound is usually a mixture of two monosulfonyl chlorides, i.e., as pictured, or with the SO3 and SO2Cl groups exchanged. It can be used as a marker of proteins, with which it easily forms conjugates via the sulfonyl chloride (SO2Cl) group. In water, the sulfonyl chloride group of unreacted Texas Red molecules hydrolyses to sulfonate and the molecule becomes the very water soluble sulforhodamine 101 which is easy to wash out selectively. This is one of the advantages of conjugating with Texas Red vs. using a rhodamine-isothiocyanate for conjugation.A protein with the Texas Red chromophore attached can then itself act as a fluorescent labelling agent; an antibody with a fluorescent marker attached will bind to a specific antigen and then show the location of the antigens as shining spots when irradiated. It is relatively bright, and therefore can be used to detect even weakly expressed antigens. Other molecules can be labeled by Texas Red as well, e.g., various toxins. The dye dissolves very well in water as well as other polar solvents, e.g., Dimethylformamide, acetonitrile.Texas Red, attached to a strand of DNA or RNA, can be used as a molecular beacon for highlighting specific sequences of DNA. Texas Red can be linked with another fluorophore. A tandem conjugate of Texas Red with R-phycoerythrin (PE-Texas Red) is often used.Fluorophores, like Texas Red, are commonly used in molecular biology techniques like quantitative RT-PCR and cellular assays.Newer rhodamine derivatives, such as Alexa 594 and DyLight 594, have been tailored to match the excitation and emission spectra of Texas Red and are used in various chemical and biological applications where greater photostability or higher fluorescence intensity are needed.".
- Texas_Red thumbnail Texas_Red.png?width=300.
- Texas_Red wikiPageID "2583840".
- Texas_Red wikiPageRevisionID "541298403".
- Texas_Red hasPhotoCollection Texas_Red.
- Texas_Red imagefile "Texas Red.png".
- Texas_Red name "Texas Red".
- Texas_Red verifiedrevid "455115567".
- Texas_Red subject Category:Fluorescent_dyes.
- Texas_Red subject Category:Rhodamine_dyes.
- Texas_Red subject Category:Staining_dyes.
- Texas_Red type AbsorptionIndicator114598383.
- Texas_Red type Abstraction100002137.
- Texas_Red type ColoringMaterial114984973.
- Texas_Red type Dye114985383.
- Texas_Red type Fluorescein114987695.
- Texas_Red type FluorescentDyes.
- Texas_Red type Indicator114917208.
- Texas_Red type Material114580897.
- Texas_Red type Matter100020827.
- Texas_Red type Part113809207.
- Texas_Red type PhysicalEntity100001930.
- Texas_Red type Relation100031921.
- Texas_Red type RhodamineDyes.
- Texas_Red type StainingDyes.
- Texas_Red type Substance100019613.
- Texas_Red type ChemicalCompound.
- Texas_Red type ChemicalSubstance.
- Texas_Red type ChemicalSubstanceType.
- Texas_Red type ChemicalObject.
- Texas_Red type Thing.
- Texas_Red comment "Texas Red or sulforhodamine 101 acid chloride is a red fluorescent dye, used in histology for staining cell specimens, for sorting cells with fluorescent-activated cell sorting machines, in fluorescence microscopy applications, and in immunohistochemistry.Texas Red fluoresces at about 615 nm, and the peak of its absorption spectrum is at 589 nm. The powder is dark purple. Solutions can be excited by a dye laser tuned to 595-605 nm, or less efficiently a krypton laser at 567 nm.".
- Texas_Red label "Texas Red".
- Texas_Red label "Texas Red".
- Texas_Red label "Texas Red".
- Texas_Red label "Texas Red".
- Texas_Red sameAs Texas_Red.
- Texas_Red sameAs Texas_Red.
- Texas_Red sameAs Texas_Red.
- Texas_Red sameAs m.07pprl.
- Texas_Red sameAs Q416900.
- Texas_Red sameAs Q416900.
- Texas_Red sameAs Texas_Red.
- Texas_Red wasDerivedFrom Texas_Red?oldid=541298403.
- Texas_Red depiction Texas_Red.png.
- Texas_Red isPrimaryTopicOf Texas_Red.
- Texas_Red name "Texas Red".