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- Yellow_fluorescent_protein abstract "Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm.Like the parent GFP, YFP is a useful tool in cell and molecular biology thanks to its properties useful for fluorescence microscopy.Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (defined as the product of the extinction coefficient and quantum yield). Typically, YFP serves as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is monomeric cyan fluorescent protein (mCFP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y substitution introduced by mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore. "Venus" contains a novel amino acid substitution –F46L– which accelerates the oxidation of the chromophore at 37°C, the rate limiting step of maturation. The protein has other substitutions (F64L/ M153T/ V163A/ S175G), permitting Venus to fold well and giving it relative tolerance to acidosis and Cl-.".
- Yellow_fluorescent_protein thumbnail Yellow_fluorescent_protein.jpg?width=300.
- Yellow_fluorescent_protein wikiPageExternalLink fpintro.html.
- Yellow_fluorescent_protein wikiPageID "4811551".
- Yellow_fluorescent_protein wikiPageRevisionID "606567704".
- Yellow_fluorescent_protein hasPhotoCollection Yellow_fluorescent_protein.
- Yellow_fluorescent_protein subject Category:Fluorescent_dyes.
- Yellow_fluorescent_protein type AbsorptionIndicator114598383.
- Yellow_fluorescent_protein type Abstraction100002137.
- Yellow_fluorescent_protein type ColoringMaterial114984973.
- Yellow_fluorescent_protein type Dye114985383.
- Yellow_fluorescent_protein type Fluorescein114987695.
- Yellow_fluorescent_protein type FluorescentDyes.
- Yellow_fluorescent_protein type Indicator114917208.
- Yellow_fluorescent_protein type Material114580897.
- Yellow_fluorescent_protein type Matter100020827.
- Yellow_fluorescent_protein type Part113809207.
- Yellow_fluorescent_protein type PhysicalEntity100001930.
- Yellow_fluorescent_protein type Relation100031921.
- Yellow_fluorescent_protein type Substance100019613.
- Yellow_fluorescent_protein comment "Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm.Like the parent GFP, YFP is a useful tool in cell and molecular biology thanks to its properties useful for fluorescence microscopy.Three improved versions of YFP are Citrine, Venus, and Ypet.".
- Yellow_fluorescent_protein label "Protéine fluorescente jaune".
- Yellow_fluorescent_protein label "Yellow fluorescent protein".
- Yellow_fluorescent_protein label "Жёлтый флуоресцентный белок".
- Yellow_fluorescent_protein sameAs Protéine_fluorescente_jaune.
- Yellow_fluorescent_protein sameAs 노랑_형광_단백질.
- Yellow_fluorescent_protein sameAs m.0cp89n.
- Yellow_fluorescent_protein sameAs Q575416.
- Yellow_fluorescent_protein sameAs Q575416.
- Yellow_fluorescent_protein sameAs Yellow_fluorescent_protein.
- Yellow_fluorescent_protein wasDerivedFrom Yellow_fluorescent_protein?oldid=606567704.
- Yellow_fluorescent_protein depiction Yellow_fluorescent_protein.jpg.
- Yellow_fluorescent_protein isPrimaryTopicOf Yellow_fluorescent_protein.