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- 01GJ028HKC4J5HVVAEKERGCCDX classification A1.
- 01GJ028HKC4J5HVVAEKERGCCDX date "2022".
- 01GJ028HKC4J5HVVAEKERGCCDX language "eng".
- 01GJ028HKC4J5HVVAEKERGCCDX type journalArticle.
- 01GJ028HKC4J5HVVAEKERGCCDX hasPart 01GJ029Y3K62TXJFZY80GWXVD6.pdf.
- 01GJ028HKC4J5HVVAEKERGCCDX subject "Veterinary Sciences".
- 01GJ028HKC4J5HVVAEKERGCCDX doi "10.3389/fvets.2022.987045".
- 01GJ028HKC4J5HVVAEKERGCCDX issn "2297-1769".
- 01GJ028HKC4J5HVVAEKERGCCDX volume "9".
- 01GJ028HKC4J5HVVAEKERGCCDX abstract "Mesenchymal stem cells (MSCs) are a promising candidate for both human and veterinary regenerative medicine applications because of their abundance and ability to differentiate into several lineages. Mesenchymal stem cells are however a heterogeneous cell population and as such, it is imperative that they are unequivocally characterized to acquire reproducible results in clinical trials. Although the tri-lineage differentiation potential of MSCs is reported in most veterinary studies, a qualitative evaluation of representative histological images does not always unambiguously confirm tri-lineage differentiation. Moreover, potential differences in differentiation capacity are not identified. Therefore, quantification of tri-lineage differentiation would greatly enhance proper characterization of MSCs. In this study, a method to quantify the tri-lineage differentiation potential of MSCs is described using digital image analysis, based on the color deconvolution plug-in (ImageJ). Mesenchymal stem cells from three species, i.e., bovine, equine, and porcine, were differentiated toward adipocytes, chondrocytes, and osteocytes. Subsequently, differentiated MSCs were stained with Oil Red O, Alcian Blue, and Alizarin Red S, respectively. Next, a differentiation ratio (DR) was obtained by dividing the area % of the differentiation signal by the area % of the nuclear signal. Although MSCs isolated from all donors in all species were capable of tri-lineage differentiation, differences were demonstrated between donors using this quantitative DR. Our straightforward, simple but robust method represents an elegant approach to determine the degree of MSC tri-lineage differentiation across species. As such, differences in differentiation potential within the heterogeneous MSC population and between different MSC sources can easily be identified, which will support further optimization of regenerative therapies.".
- 01GJ028HKC4J5HVVAEKERGCCDX author 91c6f17b-1971-11eb-b180-b1ae3d6a20e9.
- 01GJ028HKC4J5HVVAEKERGCCDX author 99ED24CC-1E7E-11E4-B0FC-3B54B5D1D7B1.
- 01GJ028HKC4J5HVVAEKERGCCDX author C40B068A-F0EE-11E1-A197-91C894A0A6B4.
- 01GJ028HKC4J5HVVAEKERGCCDX author F4E63960-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GJ028HKC4J5HVVAEKERGCCDX author F7825820-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GJ028HKC4J5HVVAEKERGCCDX author F7AF8B88-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GJ028HKC4J5HVVAEKERGCCDX dateCreated "2022-11-16T11:12:51Z".
- 01GJ028HKC4J5HVVAEKERGCCDX dateModified "2024-12-12T10:16:48Z".
- 01GJ028HKC4J5HVVAEKERGCCDX editor urn:uuid:e315c555-ab52-4df7-a026-2c2f01a06670.
- 01GJ028HKC4J5HVVAEKERGCCDX name "Validation of a color deconvolution method to quantify MSC tri-lineage differentiation across species".
- 01GJ028HKC4J5HVVAEKERGCCDX sameAs LU-01GJ028HKC4J5HVVAEKERGCCDX.
- 01GJ028HKC4J5HVVAEKERGCCDX sourceOrganization urn:uuid:17fdb0ea-ab6a-4b26-b1d8-2e00a18d71a0.
- 01GJ028HKC4J5HVVAEKERGCCDX sourceOrganization urn:uuid:89009ce0-6831-4577-ab05-5c44b5dc5a29.
- 01GJ028HKC4J5HVVAEKERGCCDX type A1.