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- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 classification A1.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 date "2022".
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 language "eng".
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 type journalArticle.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 hasPart 01GJ2QDRBTWVVGSQY8Z2HCTBWF.pdf.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 subject "Medicine and Health Sciences".
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 doi "10.1186/s12931-022-02208-1".
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 issn "1465-993X".
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 issue "1".
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 volume "23".
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 abstract "Background Two opposing B cell subsets have been defined based on their cytokine profile: IL-6 producing effector B cells (B-effs) versus IL-10 producing regulatory B cells (B-regs) that respectively positively or negatively regulate immune responses. B-regs are decreased and/or impaired in many autoimmune diseases and inflammatory conditions. Since there is increasing evidence that links B cells and B cell-rich lymphoid follicles to the pathogenesis of COPD, the aim of this study was to investigate the presence and function of B-regs in COPD. Methods First, presence of IL-10 producing regulatory B cells in human lung tissue was determined by immunohistochemistry. Secondly, quantification of IL-10 + B-regs and IL-6 + B-effs in peripheral blood mononuclear cells (PBMCs) from healthy controls, smokers without airflow limitation, and COPD patients (GOLD stage I-IV) was performed by flow cytometry. Thirdly, we exposed blood-derived B cells from COPD patients in vitro to cigarette smoke extract (CSE) and quantified IL-10 + B-regs and IL-6 + B-effs. Furthermore, we aimed at restoring the perturbed IL10 production by blocking BAFF. Fourthly, we determined mRNA expression of transcription factors involved in IL-10 production in FACS sorted memory- and naive B cells upon exposure to medium or CSE. Results The presence of IL-10 producing regulatory B cells in parenchyma and lymphoid follicles in lungs was confirmed by immunohistochemistry. The percentage of IL-10 + B-regs was significantly decreased in blood-derived memory B cell subsets from smokers without airflow limitation and patients with COPD, compared to never smokers. Furthermore, the capacity of B cells to produce IL-10 was reduced upon in vitro exposure to CSE and this could not be restored by BAFF-blockade. Finally, upon CSE exposure, mRNA levels of the transcription factors IRF4 and HIF-1 alpha, were decreased in memory B cells. Conclusion Decreased numbers and impaired function of B-regs in smokers and patients with COPD might contribute to the initiation and progression of the disease.".
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 author 100376d4-38e0-11eb-9f20-924393bb4039.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 author 106BA7D0-0464-11E3-AC31-8F7910BDE39D.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 author 29B996B4-F0EE-11E1-A9DE-61C894A0A6B4.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 author F497BC5E-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 author F5CB0EFA-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 author F640E850-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 author F86E58A6-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 dateCreated "2022-11-17T11:58:25Z".
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 dateModified "2024-12-12T17:32:50Z".
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 name "IL-10 producing regulatory B cells are decreased in blood from smokers and COPD patients".
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 pagination urn:uuid:9083ef14-e6cc-4705-8759-d62fdea522ae.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 publisher urn:uuid:821b5452-15c0-48b9-b447-85814b43d9a2.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 sameAs LU-01GJ2Q8NWA78ZHMSTZ87KA2DQ1.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 sourceOrganization urn:uuid:0a9c3ac9-7ce2-4d24-881a-4dd9be603ba3.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 sourceOrganization urn:uuid:28e60ac7-8253-4da3-8d3f-7733cfd110b4.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 sourceOrganization urn:uuid:a179d6a9-6dc5-4abc-9f21-205ab099ac6d.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 sourceOrganization urn:uuid:be8e2559-b484-4122-bc23-d0eb8e348c19.
- 01GJ2Q8NWA78ZHMSTZ87KA2DQ1 type A1.