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Ghent University Academic Bibliography

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01GJF0XZQA7JWDST6E1QDT5QEG classification A1.
01GJF0XZQA7JWDST6E1QDT5QEG date "2022".
01GJF0XZQA7JWDST6E1QDT5QEG language "eng".
01GJF0XZQA7JWDST6E1QDT5QEG type journalArticle.
01GJF0XZQA7JWDST6E1QDT5QEG hasPart 01GJF0YR0N14VC3BNVEA47B3FP.pdf.
01GJF0XZQA7JWDST6E1QDT5QEG subject "Biology and Life Sciences".
01GJF0XZQA7JWDST6E1QDT5QEG subject "Medicine and Health Sciences".
01GJF0XZQA7JWDST6E1QDT5QEG doi "10.3390/cancers14194560".
01GJF0XZQA7JWDST6E1QDT5QEG issn "2072-6694".
01GJF0XZQA7JWDST6E1QDT5QEG issue "19".
01GJF0XZQA7JWDST6E1QDT5QEG volume "14".
01GJF0XZQA7JWDST6E1QDT5QEG abstract "Simple Summary While the mouse is without doubt the most studied animal for experimental cancer research, aquatic vertebrates such as zebrafish have also contributed to the field. More recently, thanks to the Nobel-prize winning technology of CRISPR/Cas mediated genomic engineering, the frog Xenopus tropicalis has emerged as an additional powerful model for studying human cancer. Via CRISPR-mediated genome editing, several models for different human cancers have been obtained in this animal. However, what has been lacking in Xenopus is the possibility to transplant tumor cells between different frogs. This is important to allow better characterization of the tumor cells and exploration of therapeutic opportunities. In this paper, we describe the generation of a genetic mutant in Xenopus tropicalis that has a compromised immune system, thereby allowing the grafting and expansion of tumors obtained in this species. In addition, an optimized protocol is provided for the irradiation of wild-type Xenopus frogs that subsequently are temporarily immunocompromised and during that period allow tumor engraftment. This work will expand the toolbox for modeling human cancer in Xenopus tropicalis, thereby further establishing it as a powerful experimental cancer model. Modeling human genetic diseases and cancer in lab animals has been greatly aided by the emergence of genetic engineering tools such as TALENs and CRISPR/Cas9. We have previously demonstrated the ease with which genetically engineered Xenopus models (GEXM) can be generated via injection of early embryos with Cas9 recombinant protein loaded with sgRNAs targeting single or multiple tumor suppressor genes. What has been lacking so far is the possibility to propagate and characterize the induced cancers via transplantation. Here, we describe the generation of a rag2 knockout line in Xenopus tropicalis that is deficient in functional T and B cells. This line was validated by means of allografting experiments with primary tp53(-/-) and apc(+/-)/tp53(-/-) donor tumors. In addition, we optimized available protocols for the sub-lethal irradiation of wild-type X. tropicalis froglets. Irradiated animals also allowed the stable, albeit transient, engraftment of transplanted X. tropicalis tumor cells. The novel rag2(-/-) line and the irradiated wild-type froglets will further expand the experimental toolbox in the diploid amphibian X. tropicalis and help to establish it as a versatile and relevant model for exploring human cancer.".
01GJF0XZQA7JWDST6E1QDT5QEG author 05091830-F0EE-11E1-A9DE-61C894A0A6B4.
01GJF0XZQA7JWDST6E1QDT5QEG author 05A52C4E-7159-11E6-AE58-2559B5D1D7B1.
01GJF0XZQA7JWDST6E1QDT5QEG author 4B182954-2240-11E3-BD2F-D1D610BDE39D.
01GJF0XZQA7JWDST6E1QDT5QEG author 9E10BFB8-1EEF-11E2-A298-0C6810BDE39D.
01GJF0XZQA7JWDST6E1QDT5QEG author A917FA28-1676-11E3-8020-67D810BDE39D.
01GJF0XZQA7JWDST6E1QDT5QEG author C7049742-79D1-11E5-969A-8D2EB5D1D7B1.
01GJF0XZQA7JWDST6E1QDT5QEG author F593A8C0-F0ED-11E1-A9DE-61C894A0A6B4.
01GJF0XZQA7JWDST6E1QDT5QEG author F927748A-F0ED-11E1-A9DE-61C894A0A6B4.
01GJF0XZQA7JWDST6E1QDT5QEG author FA96FF34-F0ED-11E1-A9DE-61C894A0A6B4.
01GJF0XZQA7JWDST6E1QDT5QEG dateCreated "2022-11-22T06:38:13Z".
01GJF0XZQA7JWDST6E1QDT5QEG dateModified "2024-12-12T10:16:41Z".
01GJF0XZQA7JWDST6E1QDT5QEG name "Engraftment of allotransplanted tumor cells in adult rag2 mutant Xenopus tropicalis".
01GJF0XZQA7JWDST6E1QDT5QEG pagination urn:uuid:d8c0d3fc-2d77-412c-9ee7-00c33adfa201.
01GJF0XZQA7JWDST6E1QDT5QEG publisher urn:uuid:762fd18c-6e8e-4af5-ad38-34263064da5e.
01GJF0XZQA7JWDST6E1QDT5QEG sameAs LU-01GJF0XZQA7JWDST6E1QDT5QEG.
01GJF0XZQA7JWDST6E1QDT5QEG sourceOrganization urn:uuid:031339ec-50cf-40e4-a24d-7438f0d41808.
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01GJF0XZQA7JWDST6E1QDT5QEG type A1.