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- 01GKRJE20J2DX3SSSEDY3Z20AR classification A1.
- 01GKRJE20J2DX3SSSEDY3Z20AR date "2022".
- 01GKRJE20J2DX3SSSEDY3Z20AR language "eng".
- 01GKRJE20J2DX3SSSEDY3Z20AR type journalArticle.
- 01GKRJE20J2DX3SSSEDY3Z20AR hasPart 01GKRJER3N32BYE097XW5GWKA0.pdf.
- 01GKRJE20J2DX3SSSEDY3Z20AR subject "Biology and Life Sciences".
- 01GKRJE20J2DX3SSSEDY3Z20AR doi "10.1016/j.isci.2022.105311".
- 01GKRJE20J2DX3SSSEDY3Z20AR issn "2589-0042".
- 01GKRJE20J2DX3SSSEDY3Z20AR issue "11".
- 01GKRJE20J2DX3SSSEDY3Z20AR volume "25".
- 01GKRJE20J2DX3SSSEDY3Z20AR abstract "We developed a procedure for extracting maximal amounts of high-quality RNA from low-biomass producing (autotrophic) bacteria for experiments where sample volume is limited. Large amounts of high-quality RNA for downstream analyses cannot be obtained using larger quantities of culture volume. The performance of standard commercial silica-column based kit protocols and these procedures amended by ultrasonication or enzymatic lysis were assessed. The ammonium-oxidizing Nitrosomonas europaea and nitrite-oxidizing Nitrobacter winogradskyi were used as model organisms for optimization of the RNA isolation protocol. Enzymatic lysis through lysozyme digestion generated high-quality, high-yield RNA samples. Subsequent RNA-seq analysis resulted in qualitative data for both strains. The RNA extraction procedure is suitable for experiments with volume and/or biomass limitations, e.g., as encountered during space flight experiments. Furthermore, it will also result in higher RNA yields for whole transcriptome experiments where sample volume and/or biomass was increased to compensate the low-biomass characteristic of autotrophs.".
- 01GKRJE20J2DX3SSSEDY3Z20AR author 35c4add0-46d9-11ea-b040-97b025bac0df.
- 01GKRJE20J2DX3SSSEDY3Z20AR author DD7363A8-712C-11E5-A2E3-1F5AB5D1D7B1.
- 01GKRJE20J2DX3SSSEDY3Z20AR author urn:uuid:50107f0e-43d1-4914-88fe-990d1eb371ec.
- 01GKRJE20J2DX3SSSEDY3Z20AR author urn:uuid:804abe08-bcc8-486e-9144-8e00817f095b.
- 01GKRJE20J2DX3SSSEDY3Z20AR author urn:uuid:87b6b008-ce3f-405c-aee7-1349b578a249.
- 01GKRJE20J2DX3SSSEDY3Z20AR dateCreated "2022-12-08T09:52:57Z".
- 01GKRJE20J2DX3SSSEDY3Z20AR dateModified "2024-10-29T08:50:45Z".
- 01GKRJE20J2DX3SSSEDY3Z20AR name "Optimization of RNA extraction for bacterial whole transcriptome studies of low-biomass samples".
- 01GKRJE20J2DX3SSSEDY3Z20AR pagination urn:uuid:91f27b80-49c6-4e53-9593-f6a6a0762708.
- 01GKRJE20J2DX3SSSEDY3Z20AR sameAs LU-01GKRJE20J2DX3SSSEDY3Z20AR.
- 01GKRJE20J2DX3SSSEDY3Z20AR sourceOrganization urn:uuid:f8dab17f-90b7-40ba-9b87-ac4c4a617bcd.
- 01GKRJE20J2DX3SSSEDY3Z20AR type A1.