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- 01GPB2WF0KE58HP2ZKH21P5YG0 classification A1.
- 01GPB2WF0KE58HP2ZKH21P5YG0 date "2022".
- 01GPB2WF0KE58HP2ZKH21P5YG0 language "eng".
- 01GPB2WF0KE58HP2ZKH21P5YG0 type journalArticle.
- 01GPB2WF0KE58HP2ZKH21P5YG0 hasPart 01GR1EDQDM9ZNAVZ1DWX391BP2.pdf.
- 01GPB2WF0KE58HP2ZKH21P5YG0 subject "Biology and Life Sciences".
- 01GPB2WF0KE58HP2ZKH21P5YG0 doi "10.1186/s12934-022-01983-2".
- 01GPB2WF0KE58HP2ZKH21P5YG0 issn "1475-2859".
- 01GPB2WF0KE58HP2ZKH21P5YG0 issue "1".
- 01GPB2WF0KE58HP2ZKH21P5YG0 volume "21".
- 01GPB2WF0KE58HP2ZKH21P5YG0 abstract "Background: Membrane proteins (MPs) are an important class of molecules with a wide array of cellular functions and are part of many metabolic pathways. Despite their great potential-as therapeutic drug targets or in microbial cell factory optimization-many challenges remain for efficient and functional expression in a host such as Escherichia coli. Results: A dynamically regulated small RNA-based circuit was developed to counter membrane stress caused by overexpression of different MPs. The best performing small RNAs were able to enhance the maximum specific growth rate with 123%. On culture level, the total MP production was increased two-to three-fold compared to a system without dynamic control. This strategy not only improved cell growth and production of the studied MPs, it also suggested the potential use for countering metabolic burden in general. Conclusions: A dynamically regulated feedback circuit was developed that can sense metabolic stress caused by, in casu, the overexpression of an MP and responds to it by balancing the metabolic state of the cell and more specifically by downregulating the expression of the MP of interest. This negative feedback mechanism was established by implementing and optimizing simple-to-use genetic control elements based on post-transcriptional regulation: small non-coding RNAs. In addition to membrane-related stress when the MP accumulated in the cytoplasm as aggregates, the sRNA-based feedback control system was still effective for improving cell growth but resulted in a decreased total protein production. This result suggests promiscuity of the MP sensor for more than solely membrane stress.".
- 01GPB2WF0KE58HP2ZKH21P5YG0 author 1040E052-F0EE-11E1-A9DE-61C894A0A6B4.
- 01GPB2WF0KE58HP2ZKH21P5YG0 author 3770D3E4-F0EE-11E1-A9DE-61C894A0A6B4.
- 01GPB2WF0KE58HP2ZKH21P5YG0 author 3A4DB690-F0EE-11E1-A9DE-61C894A0A6B4.
- 01GPB2WF0KE58HP2ZKH21P5YG0 author 984795DA-1E7E-11E4-B0FC-3B54B5D1D7B1.
- 01GPB2WF0KE58HP2ZKH21P5YG0 author 9F407F0C-5350-11E5-9A5E-1BFBB4D1D7B1.
- 01GPB2WF0KE58HP2ZKH21P5YG0 author F6E380A6-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GPB2WF0KE58HP2ZKH21P5YG0 author F99A640E-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GPB2WF0KE58HP2ZKH21P5YG0 dateCreated "2023-01-09T10:58:10Z".
- 01GPB2WF0KE58HP2ZKH21P5YG0 dateModified "2024-11-27T13:29:35Z".
- 01GPB2WF0KE58HP2ZKH21P5YG0 name "Dynamic feedback regulation for efficient membrane protein production using a small RNA-based genetic circuit in Escherichia coli".
- 01GPB2WF0KE58HP2ZKH21P5YG0 pagination urn:uuid:56b2f643-0063-4c03-9b2d-e057ebb91b76.
- 01GPB2WF0KE58HP2ZKH21P5YG0 sameAs LU-01GPB2WF0KE58HP2ZKH21P5YG0.
- 01GPB2WF0KE58HP2ZKH21P5YG0 sourceOrganization urn:uuid:0787839b-e975-4d3f-ba04-9699ab294bbe.
- 01GPB2WF0KE58HP2ZKH21P5YG0 type A1.