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- 01GSZAGHCG1NCFA3XT30EBYJZ0 classification A1.
- 01GSZAGHCG1NCFA3XT30EBYJZ0 date "2022".
- 01GSZAGHCG1NCFA3XT30EBYJZ0 language "eng".
- 01GSZAGHCG1NCFA3XT30EBYJZ0 type journalArticle.
- 01GSZAGHCG1NCFA3XT30EBYJZ0 hasPart 01GSZAK8DDHRXF4YQ59CTJ18BF.pdf.
- 01GSZAGHCG1NCFA3XT30EBYJZ0 subject "Biology and Life Sciences".
- 01GSZAGHCG1NCFA3XT30EBYJZ0 subject "Medicine and Health Sciences".
- 01GSZAGHCG1NCFA3XT30EBYJZ0 doi "10.3791/64733".
- 01GSZAGHCG1NCFA3XT30EBYJZ0 issn "1940-087X".
- 01GSZAGHCG1NCFA3XT30EBYJZ0 issue "190".
- 01GSZAGHCG1NCFA3XT30EBYJZ0 abstract "The choroid plexus (CP), a highly vascularized structure protruding into the ventricles of the brain, is one of the most understudied tissues in neuroscience. As it is becoming increasingly clear that this tiny structure plays a crucial role in health and disease of the central nervous system (CNS), it is of utmost importance to properly dissect the CP out of the brain ventricles in a way that allows downstream processing, ranging from functional to structural analysis. Here, isolation of the lateral and fourth brain ventricle mouse CP without the need for specialized tools or equipment is described. This isolation technique preserves the viability, function, and structure of cells within the CP. On account of its high vascularization, the CP can be visualized floating inside the ventricular cavities of the brain using a binocular microscope. However, transcardial perfusion required for downstream analysis can complicate the identification of the CP tissue. Depending on the further processing steps (e.g., RNA and protein analysis), this can be solved by visualizing the CP via transcardial perfusion with bromophenol blue. After isolation, the CP can be processed using several techniques, including RNA, protein, or single cell analysis, to gain further understanding on the function of this special brain structure. Here, scanning electron microscopy (SEM) on whole mount CP is used to get an overall view of the structure.".
- 01GSZAGHCG1NCFA3XT30EBYJZ0 author 1F1D02F4-F0EE-11E1-A9DE-61C894A0A6B4.
- 01GSZAGHCG1NCFA3XT30EBYJZ0 author 30CFFDEE-F0EE-11E1-A9DE-61C894A0A6B4.
- 01GSZAGHCG1NCFA3XT30EBYJZ0 author 98261564-102D-11E3-ABFB-408B10BDE39D.
- 01GSZAGHCG1NCFA3XT30EBYJZ0 author D6E2C37A-FEE3-11E2-98EE-0C9910BDE39D.
- 01GSZAGHCG1NCFA3XT30EBYJZ0 author F589247C-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GSZAGHCG1NCFA3XT30EBYJZ0 author F6617AF2-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GSZAGHCG1NCFA3XT30EBYJZ0 dateCreated "2023-02-23T14:23:22Z".
- 01GSZAGHCG1NCFA3XT30EBYJZ0 dateModified "2024-12-12T20:40:45Z".
- 01GSZAGHCG1NCFA3XT30EBYJZ0 name "Microdissection and whole mount scanning electron microscopy visualization of mouse choroid plexus".
- 01GSZAGHCG1NCFA3XT30EBYJZ0 pagination urn:uuid:c4058e86-69ec-4e5f-9614-65cd1cbf7a78.
- 01GSZAGHCG1NCFA3XT30EBYJZ0 sameAs LU-01GSZAGHCG1NCFA3XT30EBYJZ0.
- 01GSZAGHCG1NCFA3XT30EBYJZ0 sourceOrganization urn:uuid:383aba1c-08b0-4318-8915-d18078e3807e.
- 01GSZAGHCG1NCFA3XT30EBYJZ0 sourceOrganization urn:uuid:85ff2f0c-1dde-4985-86d3-5082de7e8155.
- 01GSZAGHCG1NCFA3XT30EBYJZ0 type A1.