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- 01GVJKHN3DVVY9P68JARE18B5S classification D1.
- 01GVJKHN3DVVY9P68JARE18B5S promoter F56DD06E-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GVJKHN3DVVY9P68JARE18B5S promoter F6996D04-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GVJKHN3DVVY9P68JARE18B5S promoter F9870E72-F0ED-11E1-A9DE-61C894A0A6B4.
- 01GVJKHN3DVVY9P68JARE18B5S date "2023".
- 01GVJKHN3DVVY9P68JARE18B5S language "eng".
- 01GVJKHN3DVVY9P68JARE18B5S type dissertation.
- 01GVJKHN3DVVY9P68JARE18B5S hasPart urn:uuid:f2017510-418b-4e64-bae7-b23f56054da2.
- 01GVJKHN3DVVY9P68JARE18B5S subject "Earth and Environmental Sciences".
- 01GVJKHN3DVVY9P68JARE18B5S abstract "Safeguarding the microbial drinking water quality remains a worldwide challenge. With a growing world population, increased urbanization and climate change, the quality and availability of freshwater sources are decreasing. This, in turn, can stress the production and distribution of high-quality drinking water. Up to now, the microbiological quality of drinking water at the tap is guaranteed through the addition of chemical disinfectants that supress microbial growth in the drinking water distribution network (DWDS), such as chlorine. However, these chemicals may form potentially carcinogenic disinfection by-products, and can result in a deviating water taste and odour. Alternatively, the production of biostable water (biostability) could serve as a more sustainable approach to produce and maintain microbially safe water. In the last 40 years, researches have attempted to define and regulate biostability in different ways, which were either too complex to bring into practice, or were lacking scientific value. In Chapter 1 of this dissertation we first redefined biostability of drinking water systems as a dynamic stability of both the total microbial abundance and community composition during distribution, and we proposed a new framework for managing biostability as a balance between complexity (research) and feasibility (practice). When producing and distributing biostable drinking water, high-frequency monitoring of the microbial water quality becomes of utmost importance. In Chapter 2, we explored the use of online flow cytometry (FCM) and flow cytometric fingerprinting to monitor the biostability of drinking water systems within a full-scale water tower. We observed that events of biological instability occurred even though the water quality was legally compliant. Based on quantifying the difference between cytometric fingerprints, the Bray-Curtis dissimilarity was further developed as unambiguous parameter to indicate changes in the microbial drinking water quality during operational events. The results of Chapter 2 showed the added value of online FCM and fingerprinting as a tool for microbial water quality monitoring. In Chapter 3, we explored the applicability of a range of different online microbial monitoring techniques at a full-scale drinking water production facility. We compared their response towards operational changes and contaminations, as well as their detection limit. Enzymatic analysis, ATP measurement, and flow cytometric fingerprinting showed to be the most sensitive towards contaminations. On the other hand, optical classification and flow cytometric cell counts were more robust techniques that provide direct information about the cell concentration. These results showed that the choice for a certain technology will depend on the type of application, and will be a balance between sensitivity and maintenance. Biostability of a drinking water system implies stability of the community throughout production, the DWDS and in the household plumbing. In this regard, monitoring the microbial dynamics of domestic hot water (DHW) systems is important, as they form a potential source for the outgrowth of pathogens such as Legionella pneumophila. In Chapter 4, we investigated the dynamics of the DHW microbial abundance and community structure, and Legionella spp. in a controlled pilot system. We observed that daily hot water usage patterns and heat shock disinfection affect the microbial abundance and viability at hot water household taps and at the hot water supply. Furthermore, the results showed that Legionella spp. was not fully eradicated nor selectively enriched through heat shock disinfection. We hypothesized that selecting for heat-resistant species in the microbiome may be an alternative approach to maintain a biostable microbiome within DHW environments. Based on the theoretical framework developed in Chapter 1, the hypothesis was formulated that biostability can be accomplished through the creation of an environment that is oligotrophic in all available nutrients but eutrophic in energy. More specifically, the water can be “energized” through addition of hydrogen gas (H2) and oxygen gas (O2) as electron donor and acceptor, respectively. This way, autotrophic growth of hydrogen-oxidizing bacteria (HOB) is stimulated. In Chapter 5, we explored the potential of HOB for the production of biostable drinking water in a continuous trickling filter supplied with hydrogen gas. The bacterial regrowth, invasion potential, and nutrient composition of the water were determined. Treatment showed to improve the biostability significantly, and it is hypothesized that nutrient limitation, especially phosphorous, was a driving force. As a result, the regrowth and invasion potential were lowered, as shown with specific biostability bioassays. Overall, these results demonstrated the effectiveness of HOB for producing biostable drinking water through nutrient limitation. In conclusion, understanding of microbial (re)growth and its driving factors during production, storage, in the DWDS and household plumbing remain of utmost importance to ensure the microbial water quality from source to tap. In this respect, producing biostable drinking water, combined with online monitoring of the microbial dynamics, implemented within a network-specific microbial water quality management framework are the way forward for safeguarding the drinking water quality. In Chapter 6 of this dissertation, the insights gained from the research performed as a part of this dissertation are combined and discussed within a broader context of practical applications, future research challenges and the legislative framework.".
- 01GVJKHN3DVVY9P68JARE18B5S author 8F313752-14E4-11E3-822E-DD8B10BDE39D.
- 01GVJKHN3DVVY9P68JARE18B5S dateCreated "2023-03-15T12:22:32Z".
- 01GVJKHN3DVVY9P68JARE18B5S dateModified "2024-10-29T08:53:04Z".
- 01GVJKHN3DVVY9P68JARE18B5S name "Biostability of drinking water systems : from online monitoring to microbial quality management".
- 01GVJKHN3DVVY9P68JARE18B5S pagination urn:uuid:94f32f19-898b-43fa-aa91-3fabf2a24919.
- 01GVJKHN3DVVY9P68JARE18B5S publisher urn:uuid:e4fbc215-3d03-4c71-ac60-cce655fc6390.
- 01GVJKHN3DVVY9P68JARE18B5S sameAs LU-01GVJKHN3DVVY9P68JARE18B5S.
- 01GVJKHN3DVVY9P68JARE18B5S sourceOrganization urn:uuid:074dfe02-292b-4bfc-8d6b-00667bc25516.
- 01GVJKHN3DVVY9P68JARE18B5S sourceOrganization urn:uuid:57d64d29-5675-40e5-bedf-1e70755765f1.
- 01GVJKHN3DVVY9P68JARE18B5S type D1.