Matches in Ghent University Academic Bibliography for { <https://biblio.ugent.be/publication/01H6RDJKV3ME471AVZCHBAV2F6> ?p ?o. }
Showing items 1 to 28 of
28
with 100 items per page.
- 01H6RDJKV3ME471AVZCHBAV2F6 classification A1.
- 01H6RDJKV3ME471AVZCHBAV2F6 date "2023".
- 01H6RDJKV3ME471AVZCHBAV2F6 language "eng".
- 01H6RDJKV3ME471AVZCHBAV2F6 type journalArticle.
- 01H6RDJKV3ME471AVZCHBAV2F6 hasPart 01H6RER629CYKEGNT8GA78EK1E.pdf.
- 01H6RDJKV3ME471AVZCHBAV2F6 hasPart 01H6REXRDF51CNGHAAKGCGGG24.pdf.
- 01H6RDJKV3ME471AVZCHBAV2F6 subject "Medicine and Health Sciences".
- 01H6RDJKV3ME471AVZCHBAV2F6 doi "10.3390/ijms241411612".
- 01H6RDJKV3ME471AVZCHBAV2F6 issn "1422-0067".
- 01H6RDJKV3ME471AVZCHBAV2F6 issn "1661-6596".
- 01H6RDJKV3ME471AVZCHBAV2F6 issue "14".
- 01H6RDJKV3ME471AVZCHBAV2F6 volume "24".
- 01H6RDJKV3ME471AVZCHBAV2F6 abstract "Cx43 hemichannels (HCs) and Panx1 channels are two genetically distant protein families. Despite the lack of sequence homology, Cx43 and Panx1 channels have been the subject of debate due to their overlapping expression and the fact that both channels present similarities in terms of their membrane topology and electrical properties. Using the mimetic peptides Gap19 and 10Panx1, this study aimed to investigate the cross-effects of these peptides on Cx43 HCs and Panx1 channels. The single-channel current activity from stably expressing HeLa-Cx43 and C6-Panx1 cells was recorded using patch-clamp experiments in whole-cell voltage-clamp mode, demonstrating 214 pS and 68 pS average unitary conductances for the respective channels. Gap19 was applied intracellularly while 10Panx1 was applied extracellularly at different concentrations (100, 200 and 500 μM) and the average nominal open probability (NPo) was determined for each testing condition. A concentration of 100 µM Gap19 more than halved the NPo of Cx43 HCs, while 200 µM 10Panx1 was necessary to obtain a half-maximal NPo reduction in the Panx1 channels. Gap19 started to significantly inhibit the Panx1 channels at 500 µM, reducing the NPo by 26% while reducing the NPo of the Cx43 HCs by 84%. In contrast 10Panx1 significantly reduced the NPo of the Cx43 HCs by 37% at 100 µM and by 83% at 200 µM, a concentration that caused the half-maximal inhibition of the Panx1 channels. These results demonstrate that 10Panx1 inhibits Cx43 HCs over the 100–500 µM concentration range while 500 µM intracellular Gap19 is necessary to observe some inhibition of Panx1 channels.".
- 01H6RDJKV3ME471AVZCHBAV2F6 author 2AD342B0-DBD0-11E8-96C2-E4685607D3EF.
- 01H6RDJKV3ME471AVZCHBAV2F6 author 2CD6B818-C067-11E4-8E67-6B8BB5D1D7B1.
- 01H6RDJKV3ME471AVZCHBAV2F6 author 5F0717AE-DFE0-11E9-B4DE-7A315707D3EF.
- 01H6RDJKV3ME471AVZCHBAV2F6 author F0912938-2E35-11E4-BFAF-1ECAB4D1D7B1.
- 01H6RDJKV3ME471AVZCHBAV2F6 author F44FA202-F0ED-11E1-A9DE-61C894A0A6B4.
- 01H6RDJKV3ME471AVZCHBAV2F6 dateCreated "2023-08-01T11:26:18Z".
- 01H6RDJKV3ME471AVZCHBAV2F6 dateModified "2024-12-12T19:38:06Z".
- 01H6RDJKV3ME471AVZCHBAV2F6 editor urn:uuid:02d39ca6-0458-4d4f-8b76-6a876306c20c.
- 01H6RDJKV3ME471AVZCHBAV2F6 editor urn:uuid:65af935c-fd0d-441f-b2e8-fdf33657fb83.
- 01H6RDJKV3ME471AVZCHBAV2F6 name "Cx43 hemichannel and Panx1 channel modulation by Gap19 and 10Panx1 peptides".
- 01H6RDJKV3ME471AVZCHBAV2F6 pagination urn:uuid:77244bd8-31f0-48e1-a95b-1d966e7c13fb.
- 01H6RDJKV3ME471AVZCHBAV2F6 publisher urn:uuid:0f07fa03-bc8d-4988-804b-7c572e369b5e.
- 01H6RDJKV3ME471AVZCHBAV2F6 sameAs LU-01H6RDJKV3ME471AVZCHBAV2F6.
- 01H6RDJKV3ME471AVZCHBAV2F6 sourceOrganization urn:uuid:bbea8945-8592-4663-884d-c38d2b86c98e.
- 01H6RDJKV3ME471AVZCHBAV2F6 type A1.