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- 01H847S58J4KV4G95S0BZSJSMK classification A1.
- 01H847S58J4KV4G95S0BZSJSMK date "2023".
- 01H847S58J4KV4G95S0BZSJSMK language "eng".
- 01H847S58J4KV4G95S0BZSJSMK type journalArticle.
- 01H847S58J4KV4G95S0BZSJSMK hasPart 01H847WJPG0ZARF4Z21VRPNZG8.pdf.
- 01H847S58J4KV4G95S0BZSJSMK subject "Biology and Life Sciences".
- 01H847S58J4KV4G95S0BZSJSMK doi "10.1371/journal.pone.0269717".
- 01H847S58J4KV4G95S0BZSJSMK issn "1932-6203".
- 01H847S58J4KV4G95S0BZSJSMK issue "8".
- 01H847S58J4KV4G95S0BZSJSMK volume "18".
- 01H847S58J4KV4G95S0BZSJSMK abstract "Carlsberg subtilisin from Bacillus licheniformis PB1 was investigated as a potential feed supplement, through immobilizing on bentonite for improving the growth rate of broilers. Initially, the pre-optimized and partially-purified protease was extracted and characterized using SDS-PAGE with MW 27.0 KDa. The MALDI-TOF-MS/MS spectrum confirmed a tryptic peptide peak with m/z 1108.496 referring to the Carlsberg subtilisin as a protein-digesting enzyme with alkaline nature. The highest free enzyme activity (30 U/mg) was observed at 50 & DEG;C, 1 M potassium phosphate, and pH 8.0. the enhanced stability was observed when the enzyme was adsorbed to an inert solid support with 86.39 & PLUSMN; 4.36% activity retention under 20 optimized conditions. Additionally, the dried immobilized enzyme exhibited only a 5% activity loss after two-week storage at room temperature. Structural modeling (Docking) revealed that hydrophobic interactions between bentonite and amino acids surrounding the catalytic triad keep the enzyme structure intact upon drying at RT. The prominent hygroscopic nature of bentonite facilitated protein structure retention upon drying. During a 46-days study, supplementation of boilers' feed with the subtilisin-bentonite complex promoted significant weight gain i.e. 15.03% in contrast to positive control (p = 0.001).".
- 01H847S58J4KV4G95S0BZSJSMK author 1FC9EA8C-F0EE-11E1-A9DE-61C894A0A6B4.
- 01H847S58J4KV4G95S0BZSJSMK author F4B312A6-F0ED-11E1-A9DE-61C894A0A6B4.
- 01H847S58J4KV4G95S0BZSJSMK author urn:uuid:0a59ea02-c94a-48c9-9932-88f8838d4765.
- 01H847S58J4KV4G95S0BZSJSMK author urn:uuid:14916ace-bbfd-42fd-8b3d-eeda095d502e.
- 01H847S58J4KV4G95S0BZSJSMK author urn:uuid:49569536-47c5-4d98-94a5-a8d8dc73b601.
- 01H847S58J4KV4G95S0BZSJSMK author urn:uuid:a7952bba-5c0a-432e-aab8-66b97dc8ace3.
- 01H847S58J4KV4G95S0BZSJSMK author urn:uuid:be99f591-af83-4413-932a-e2c09fdb836e.
- 01H847S58J4KV4G95S0BZSJSMK author urn:uuid:dc0d3451-31e9-4ad2-97b7-2bdeea6105d8.
- 01H847S58J4KV4G95S0BZSJSMK dateCreated "2023-08-18T11:51:36Z".
- 01H847S58J4KV4G95S0BZSJSMK dateModified "2024-12-12T17:57:58Z".
- 01H847S58J4KV4G95S0BZSJSMK editor urn:uuid:2af3938b-f9ad-40cb-99cc-dd2ac0d2ea0c.
- 01H847S58J4KV4G95S0BZSJSMK name "Immobilization and docking studies of Carlsberg subtilisin for application in poultry industry".
- 01H847S58J4KV4G95S0BZSJSMK pagination urn:uuid:bd8b903e-32d8-4b85-bccb-838602d0069a.
- 01H847S58J4KV4G95S0BZSJSMK sameAs LU-01H847S58J4KV4G95S0BZSJSMK.
- 01H847S58J4KV4G95S0BZSJSMK sourceOrganization urn:uuid:7c692c37-3e3b-4fed-bf8c-27474cf19acb.
- 01H847S58J4KV4G95S0BZSJSMK type A1.