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- 01HDDTSF58GN2TTF9DFRFYTEQ5 classification C3.
- 01HDDTSF58GN2TTF9DFRFYTEQ5 date "2022".
- 01HDDTSF58GN2TTF9DFRFYTEQ5 language "eng".
- 01HDDTSF58GN2TTF9DFRFYTEQ5 type conference.
- 01HDDTSF58GN2TTF9DFRFYTEQ5 subject "Biology and Life Sciences".
- 01HDDTSF58GN2TTF9DFRFYTEQ5 subject "Earth and Environmental Sciences".
- 01HDDTSF58GN2TTF9DFRFYTEQ5 subject "Technology and Engineering".
- 01HDDTSF58GN2TTF9DFRFYTEQ5 presentedAt urn:uuid:38cf9660-3472-4cfb-b601-c1a50dd15e4e.
- 01HDDTSF58GN2TTF9DFRFYTEQ5 abstract "Klebsiella pneumoniae has been labeled as an 'ESKAPE' organism by the WHO, due to its growing multidrug resistance and virulence, urgently requiring antibiotic alternatives. Klebsiella phages are highly specific against their host bacterium, recognizing the capsule serotype with their receptor-binding proteins (RBPs). To overcome limited host specificity in traditional phage therapy, we elaborate a synthetic phage framework in which a diversity of receptor-binding proteins (RBPs) can be plugged in. The bivalent Klebsiella phage KP32 is used as a scaffold and possesses a dual RBP system with an anchor-branched structure. This branched RBP system can be divided into the following modular building blocks: a N-terminal anchor for attachment of the first RBP to the phage tail, including a T4gp10-like branching domain; a different C-terminal specificity domain with depolymerase activity for the first and second RBP, respectively, and a conserved peptide to attach the C-terminal specificity domain of the second RBP to the T4gp10-like branching domain of the first RBP. Using the VersaTile technique as a modular DNA assembly technique, a collection of modular building blocks derived from different Klebsiella phages (KP32, K11, KP34 and KP36) were assembled, resulting in chimeric RBP gene clusters, that were subsequently integrated into the well-characterized phage scaffold of KP32. In the future, this strategy may allow us to create customized phages, consisting of a safe and well-known phage scaffold in which specificity to the bacterial host can be reprogrammed by only changing the RBPs targeting the patient-specific bacterial pathogen.".
- 01HDDTSF58GN2TTF9DFRFYTEQ5 author 75BE61A6-8759-11E6-8E0E-8233B5D1D7B1.
- 01HDDTSF58GN2TTF9DFRFYTEQ5 author C157F57C-AA66-11E4-986E-18D2B4D1D7B1.
- 01HDDTSF58GN2TTF9DFRFYTEQ5 author C1723F58-E687-11E2-AD28-C2B310BDE39D.
- 01HDDTSF58GN2TTF9DFRFYTEQ5 author D7711A38-79FD-11E6-A1F1-3577B5D1D7B1.
- 01HDDTSF58GN2TTF9DFRFYTEQ5 author urn:uuid:864864cc-db46-4a1b-a6e1-2f929a7e5dea.
- 01HDDTSF58GN2TTF9DFRFYTEQ5 dateCreated "2023-10-23T08:35:29Z".
- 01HDDTSF58GN2TTF9DFRFYTEQ5 dateModified "2024-10-29T18:40:02Z".
- 01HDDTSF58GN2TTF9DFRFYTEQ5 name "Development of a synthetic, VersaTile strategy for receptor-binding protein engineering of Klebsiella pneumoniae phages".
- 01HDDTSF58GN2TTF9DFRFYTEQ5 sameAs LU-01HDDTSF58GN2TTF9DFRFYTEQ5.
- 01HDDTSF58GN2TTF9DFRFYTEQ5 sourceOrganization urn:uuid:991bc0cd-85d7-4e6c-9415-8fcb10a38340.
- 01HDDTSF58GN2TTF9DFRFYTEQ5 type C3.