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- 01HG8FYCDGYY4K4MGJDK0ATBYT classification C3.
- 01HG8FYCDGYY4K4MGJDK0ATBYT date "2023".
- 01HG8FYCDGYY4K4MGJDK0ATBYT language "eng".
- 01HG8FYCDGYY4K4MGJDK0ATBYT type conference.
- 01HG8FYCDGYY4K4MGJDK0ATBYT hasPart 01HG8S9AW1RNNCMH2XJ2X8ABVK.pdf.
- 01HG8FYCDGYY4K4MGJDK0ATBYT subject "Medicine and Health Sciences".
- 01HG8FYCDGYY4K4MGJDK0ATBYT presentedAt urn:uuid:b83511da-1f5a-4b08-aff1-0125f402457c.
- 01HG8FYCDGYY4K4MGJDK0ATBYT abstract "Human biomonitoring, which is the direct measurement of biomarkers of exposure and effect in biological fluids, is considered an efficient way to assess human exposure to mycotoxins. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) has become a powerful tool for both quantitative and qualitative analysis of multiple mycotoxins in various matrices, but there is a need for high-throughput, selective and sensitive methods for limited sample volumes. We have developed a methodology for suspect screening of 105 mycotoxins and metabolites in human serum by UHPLC-high resolution MS/MS followed by data analysis using UNIFI software (Waters®). UNIFI was used to perform automatic peak processing and subsequent confirmation of compound identity employing an in-house library containing MS/MS information found in the literature as well as the retention time, experimentally determined when commercial standards were available or predicted with retention time prediction Retip R package in absence of standards. The method was validated by spiking standards of 40 mycotoxins and metabolites in human serum and the limit of detection (LOD) and lower limit of quantification (LLOQ) ranged from 1.1 to 30 µg/L and 2.0 to 55 µg/L, respectively. Compared to targeted analysis using multiple reaction monitoring, the suspect screening method had a LOD and LLOQ about 10 times higher for most mycotoxins. However, targeted analysis was limited by the availability of standards. Conversely, the screening method did not require standards for qualitative analysis, allowing detection of the mycotoxins and metabolites included in the library and retrospective data analysis if more compounds or information are added to the library in the future. The developed suspect screening and targeted methods were applied to human serum samples from Ethiopia, in which 7 different mycotoxins were detected, including citrinin, deoxynivalenol, ochratoxin A and tenuazonic acid.".
- 01HG8FYCDGYY4K4MGJDK0ATBYT author 0BAC47AC-F0EE-11E1-A9DE-61C894A0A6B4.
- 01HG8FYCDGYY4K4MGJDK0ATBYT author 72ddfc9e-860e-11ec-bc8a-f8607f666504.
- 01HG8FYCDGYY4K4MGJDK0ATBYT author EEB056EA-7AC6-11E6-962D-B4E9B4D1D7B1.
- 01HG8FYCDGYY4K4MGJDK0ATBYT author F4CD454A-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HG8FYCDGYY4K4MGJDK0ATBYT author af4d77b1-aac8-11eb-8deb-f53b156d7cda.
- 01HG8FYCDGYY4K4MGJDK0ATBYT author urn:uuid:075b140c-a2c2-4945-80a6-a65c3c130727.
- 01HG8FYCDGYY4K4MGJDK0ATBYT author urn:uuid:23ff71da-fc9b-47ae-982f-f37f99c37949.
- 01HG8FYCDGYY4K4MGJDK0ATBYT author urn:uuid:3c7f528d-a166-416b-a9d3-d0be15232290.
- 01HG8FYCDGYY4K4MGJDK0ATBYT dateCreated "2023-11-27T13:36:49Z".
- 01HG8FYCDGYY4K4MGJDK0ATBYT dateModified "2024-12-12T19:17:55Z".
- 01HG8FYCDGYY4K4MGJDK0ATBYT name "Potential of liquid chromatography-mass spectrometry for suspect screening and targeted analysis of multiple mycotoxin exposure in human serum".
- 01HG8FYCDGYY4K4MGJDK0ATBYT pagination urn:uuid:3693bbfa-46e7-420a-b1ba-97e4a8110178.
- 01HG8FYCDGYY4K4MGJDK0ATBYT sameAs LU-01HG8FYCDGYY4K4MGJDK0ATBYT.
- 01HG8FYCDGYY4K4MGJDK0ATBYT sourceOrganization urn:uuid:776111c1-6cae-4e8a-92e6-5f0bb54bbbcf.
- 01HG8FYCDGYY4K4MGJDK0ATBYT type C3.