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- 01HGD7MPF697FPG2HT17VWFSV8 classification C3.
- 01HGD7MPF697FPG2HT17VWFSV8 date "2023".
- 01HGD7MPF697FPG2HT17VWFSV8 language "eng".
- 01HGD7MPF697FPG2HT17VWFSV8 type conference.
- 01HGD7MPF697FPG2HT17VWFSV8 hasPart 01HGD7R2C3WE7B93BG8B9QBEZE.pdf.
- 01HGD7MPF697FPG2HT17VWFSV8 subject "Earth and Environmental Sciences".
- 01HGD7MPF697FPG2HT17VWFSV8 subject "Technology and Engineering".
- 01HGD7MPF697FPG2HT17VWFSV8 presentedAt urn:uuid:ff57df92-3c56-4e3b-8f6b-40c86ff7dbbc.
- 01HGD7MPF697FPG2HT17VWFSV8 abstract "Circular economy is becoming a more important part of our daily life, as we need to move towards a greener, more sustainable future. The seafood, fungi and insect industry create high amounts of chitin-rich waste. Chitin can be broken down to the monomer N-acetylglucosamine (GlcNAc), which can serve as a carbon source for Escherichia coli in the biotechnological production of value-added products such as chitooligosaccharides (COS). E. coli can convert GlcNAc to UDP-GlcNAc via its native hexosamine biosynthesis pathway (HBP). With the addition of a chitin synthase, COS can be produced starting from UDP-GlcNAc. To maximize production, a double carbon source was used, glycerol for growth and GlcNAc for production. However, when engineering the strains to optimize the flow to UDP-GlcNAc and prevent GlcNAc from enter the central metabolism, the strains immediately cease growth. In this research, the so-called amino sugar-P stress is studied to get more fundamental insight in the triggers and regulatory mechanisms and in addition provide us with engineering opportunities to redirect all incoming GlcNAc to COS production. A multi-omics approach was used to enable this and metabolomics already revealed metabolites correlated with the changed growth profile after GlcNAc addition. Based on the knowledge gained from the metabolomics analysis, different engineering strategies, both static and dynamic, are currently being tested for their ability to relieve the cells from stress and improve COS production starting from GlcNAc. In addition, a transcriptomics analysis is being conducted to uncover the underlying stress responses. Together with the metabolomics data this will give us a better understanding of amino sugar-P stress. This research thus contributes to fundamental knowledge about microbial stress and delivers stress-resistant strains, that can be deployed for the valorisation of waste streams enabling the transition to a circular economy and a more responsible production process.".
- 01HGD7MPF697FPG2HT17VWFSV8 author 3A4DB690-F0EE-11E1-A9DE-61C894A0A6B4.
- 01HGD7MPF697FPG2HT17VWFSV8 author 67AF3900-A269-11E8-9B1B-51D55607D3EF.
- 01HGD7MPF697FPG2HT17VWFSV8 author 6E467E86-2BDA-11E4-ADE1-00BDB4D1D7B1.
- 01HGD7MPF697FPG2HT17VWFSV8 author F99A640E-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HGD7MPF697FPG2HT17VWFSV8 author urn:uuid:2c6b0eff-5d64-496e-8507-863069bad5cf.
- 01HGD7MPF697FPG2HT17VWFSV8 author urn:uuid:525446b3-4d89-439e-bead-70bbcc030b56.
- 01HGD7MPF697FPG2HT17VWFSV8 dateCreated "2023-11-29T09:47:55Z".
- 01HGD7MPF697FPG2HT17VWFSV8 dateModified "2024-10-29T17:38:28Z".
- 01HGD7MPF697FPG2HT17VWFSV8 name "Multi-omics approach for the valorisation of chitin-rich waste into specialty sugars using E. coli".
- 01HGD7MPF697FPG2HT17VWFSV8 sameAs LU-01HGD7MPF697FPG2HT17VWFSV8.
- 01HGD7MPF697FPG2HT17VWFSV8 sourceOrganization urn:uuid:4412c84b-e053-4079-bf23-0ff20ab6c9a5.
- 01HGD7MPF697FPG2HT17VWFSV8 type C3.