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- 01HMGZGGXKHMXV3YJM1B4ZRDPP classification C3.
- 01HMGZGGXKHMXV3YJM1B4ZRDPP date "2023".
- 01HMGZGGXKHMXV3YJM1B4ZRDPP language "eng".
- 01HMGZGGXKHMXV3YJM1B4ZRDPP type conference.
- 01HMGZGGXKHMXV3YJM1B4ZRDPP hasPart 01HMGZVSGZW8VC49X1QXCWNTSP.pdf.
- 01HMGZGGXKHMXV3YJM1B4ZRDPP subject "Veterinary Sciences".
- 01HMGZGGXKHMXV3YJM1B4ZRDPP presentedAt urn:uuid:47627bd6-ffc3-4d85-9fd7-69a11522ba6d.
- 01HMGZGGXKHMXV3YJM1B4ZRDPP abstract "Exuberant granulation tissue (EGT) is a disorder of second intention wound healing at distal limbs of horses. EGT is characterized by hyperproliferating granulation tissue. During the proliferative phase, myofibroblasts produce the immature collagen III which is later replaced by the mature collagen I in the remodeling phase. Here, we investigated the collagen deposition and maturation in EGT versus experimentally induced wounds. According to the fibers’ thickness, absorbance and their birefringence, Picrosirius red staining enables differentiation of green, immature and yellow-red, mature collagen fibers under polarized light. Archived formalin-fixed paraffin- embedded biopsies were used from a previous study approved by the Ghent University ethical committee (approval number 2014/183). In total, 12 clinical cases displaying EGT and eight experimentally induced wounds were analyzed at different sampling time points. Picrosirius red staining was analyzed with QuPath. We quantified the ratio of green vs. red fibers which facilitates the creation of groups of early (up to 14 days old) and late stages (more than 14 days old) of wounds based on the clinical report of wound age. Both in the experimental and the field samples, the average ratio of immature to mature collagen of EGT wounds at early stages is higher than in older ones (early: X ̅ = 0,67 [±0,56]; late: X ̅ = 0,32 [±0,49]), which is equally in the experimental induced wounds (early: X ̅ = 0,7549 [±0,1446]; late: X ̅ = 0,228 [±0,1603]). A microscopical affirmation of the previously reported age of the EGT wound based on the range of the control wounds was successful in seven samples. In summary, collagen remodeling in EGT mostly resembles experimentally induced wounds, with the difference that the latter were performed under almost sterile conditions. The next step of this ongoing work is to substantiate our findings by investigating more sample".
- 01HMGZGGXKHMXV3YJM1B4ZRDPP author 19fbe2cc-aa34-11ec-9859-8804b9a71f2a.
- 01HMGZGGXKHMXV3YJM1B4ZRDPP author F87618B6-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HMGZGGXKHMXV3YJM1B4ZRDPP author urn:uuid:2543c1b5-6e15-46f2-9cf0-40d92c2c2e83.
- 01HMGZGGXKHMXV3YJM1B4ZRDPP dateCreated "2024-01-19T13:45:34Z".
- 01HMGZGGXKHMXV3YJM1B4ZRDPP dateModified "2024-10-29T18:35:22Z".
- 01HMGZGGXKHMXV3YJM1B4ZRDPP name "Collagen remodeling in equine exuberant granulation tissue characterized by Picrosirius Red staining".
- 01HMGZGGXKHMXV3YJM1B4ZRDPP pagination urn:uuid:2a3e8a0d-f13d-4137-8808-032d060a9df7.
- 01HMGZGGXKHMXV3YJM1B4ZRDPP sameAs LU-01HMGZGGXKHMXV3YJM1B4ZRDPP.
- 01HMGZGGXKHMXV3YJM1B4ZRDPP sourceOrganization urn:uuid:4038111b-8104-45c5-a302-d6ecdb8edb69.
- 01HMGZGGXKHMXV3YJM1B4ZRDPP type C3.