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- 01HPHV8RXRNHD6XVM52K85P5JH classification C3.
- 01HPHV8RXRNHD6XVM52K85P5JH date "2022".
- 01HPHV8RXRNHD6XVM52K85P5JH language "eng".
- 01HPHV8RXRNHD6XVM52K85P5JH type conference.
- 01HPHV8RXRNHD6XVM52K85P5JH hasPart 01HPHVDVDVVZC97AC2VAPQDCA9.pdf.
- 01HPHV8RXRNHD6XVM52K85P5JH subject "Biology and Life Sciences".
- 01HPHV8RXRNHD6XVM52K85P5JH presentedAt urn:uuid:43382933-8775-4306-94ef-dcd13dad2d57.
- 01HPHV8RXRNHD6XVM52K85P5JH abstract "Microbial cell factories have great potential to produce valuable products such as biofuels, materials, pharmaceuticals and chemicals from renewable feedstocks, or even waste materials, there-by facilitating transition to a circular economy and achievement of UN sustainable development goals. The bacterial domain contains a huge natural diversity, from extremophiles to native producers, that could be exploited as microbial cell factory hosts. However, this is currently limited by a lack of well characterised and extensive tools for regulating gene expression, especially in non-model organisms. To utilise the full potential of these organisms, this research aims to develop and characterise a cross-bacterial, orthogonal and tuneable gene expression system, allowing predictable engineering in diverse bacterial hosts. Orthogonality is key in achieving this aim, allowing expression of the gene of interest independently from the host metabolism, and therefore reducing environmental interference. Here we show the use of rational DNA libraries and a highly sensitive screening method to find promoters that are orthogonal to the host metabolism. These promoter sequences are the starting point for development of the gene expression toolbox, from which transcription will be regulated by an engineered transcription initiation factor. Such a genetic toolbox is a key enabling technology in industrial biotechnology as it will facilitate selection of the optimum microorganism for the application, greatly reducing process development times and costs and reducing time to market.".
- 01HPHV8RXRNHD6XVM52K85P5JH author 1040E052-F0EE-11E1-A9DE-61C894A0A6B4.
- 01HPHV8RXRNHD6XVM52K85P5JH author 1ed3b124-1c96-11eb-8a4d-93dc5bbb8855.
- 01HPHV8RXRNHD6XVM52K85P5JH author 3770D3E4-F0EE-11E1-A9DE-61C894A0A6B4.
- 01HPHV8RXRNHD6XVM52K85P5JH author F99A640E-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HPHV8RXRNHD6XVM52K85P5JH author urn:uuid:db50c4c1-9711-44ab-a65c-988fe292988d.
- 01HPHV8RXRNHD6XVM52K85P5JH dateCreated "2024-02-13T18:22:04Z".
- 01HPHV8RXRNHD6XVM52K85P5JH dateModified "2024-10-29T18:40:07Z".
- 01HPHV8RXRNHD6XVM52K85P5JH name "A cross-bacterial, orthogonal and tuneable gene expression toolbox for development of microbial cell factories in diverse bacterial hosts".
- 01HPHV8RXRNHD6XVM52K85P5JH sameAs LU-01HPHV8RXRNHD6XVM52K85P5JH.
- 01HPHV8RXRNHD6XVM52K85P5JH sourceOrganization urn:uuid:d5ae1580-dab4-45e7-907b-9108f8f4c462.
- 01HPHV8RXRNHD6XVM52K85P5JH type C3.