Matches in Ghent University Academic Bibliography for { <https://biblio.ugent.be/publication/01HQR1TT2MPJJ7JCYHWAC0KJDD> ?p ?o. }
Showing items 1 to 21 of
21
with 100 items per page.
- 01HQR1TT2MPJJ7JCYHWAC0KJDD classification C3.
- 01HQR1TT2MPJJ7JCYHWAC0KJDD date "2024".
- 01HQR1TT2MPJJ7JCYHWAC0KJDD language "eng".
- 01HQR1TT2MPJJ7JCYHWAC0KJDD type conference.
- 01HQR1TT2MPJJ7JCYHWAC0KJDD subject "Biology and Life Sciences".
- 01HQR1TT2MPJJ7JCYHWAC0KJDD subject "Medicine and Health Sciences".
- 01HQR1TT2MPJJ7JCYHWAC0KJDD subject "Veterinary Sciences".
- 01HQR1TT2MPJJ7JCYHWAC0KJDD presentedAt urn:uuid:faf5f00f-012f-4609-8847-03c37054fb58.
- 01HQR1TT2MPJJ7JCYHWAC0KJDD abstract "Problem: Several challenges account for the slow progress of cultured meat production. Primary bovine muscle stem cells have limited cell expansion potential, lose their myogenic differentiation capacity to myotubes after prolonged expansion and the resulting myotubes are immature. The finite pool of myoblasts and the absence of well-structured myofibers hinder the efficient and scalable production of cultured meat. Objective: To enhance the myogenic differentiation of primary-derived myosatellite cells, we explore the effect of stimulation on cultured bovine myoblasts during cell expansion and myotube formation. Methodology: We evaluate the effect of two different stimulation techniques: pulsed-electromagnetic field stimulation and mechanical stimulation on bovine myoblasts during proliferation as well as differentiation. We investigate the impact of stimulation on the cell number, fusion capacity, cell alignment, myotube diameter, polynucleation, metabolic activity and sarcomere component expression during myogenesis in 2D as well as 3D. In addition, for 3D, mechanical characterization is performed. Results: Continuous and intermitted pulsed electromagnetic field stimulation is applied to high- and low-fusion performing bovine myoblasts in 2D. The results demonstrate that the stimulated myoblasts have an increased fusion capacity compared to statically cultured cells, with enhanced myotube formation in low-fusion myoblasts due to the stimulation. Moreover, an increased metabolic activity (7 ± 3%) is observed in the 2D cultured myoblasts after stimulation, indicating a positive impact on cellular function. Mechanical stimulation is tested during the differentiation of 3D muscle constructs of myoblast donors with low-fusion characteristics. The stimulation results in a decrease in Young’s modulus (43 ± 4% kPa), indicating that the stimulated tissues are less stiff. Additionally, the stimulation induces an increase in metabolic activity (75 ± 10%) and an elevated fusion capacity, collectively suggesting improved myoblast myogenesis. Conclusion: This research highlights the potential of stimulating bovine myoblasts to enhance myogenesis, thereby increasing or partially restoring the myogenic fusion capacity. These findings contribute toward overcoming the challenges of immature bovine myoblasts, paving the way for the development of muscle constructs with application to cultured meat production.".
- 01HQR1TT2MPJJ7JCYHWAC0KJDD author 0aaf9d91-1c96-11eb-8a4d-cd3f2d191e5a.
- 01HQR1TT2MPJJ7JCYHWAC0KJDD author 91c6f17b-1971-11eb-b180-b1ae3d6a20e9.
- 01HQR1TT2MPJJ7JCYHWAC0KJDD author F7825820-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HQR1TT2MPJJ7JCYHWAC0KJDD author ca408869-8c57-11ec-ab6d-c999412fbb21.
- 01HQR1TT2MPJJ7JCYHWAC0KJDD author urn:uuid:94025380-8d35-474c-aa07-80fde157dadf.
- 01HQR1TT2MPJJ7JCYHWAC0KJDD dateCreated "2024-02-28T14:27:55Z".
- 01HQR1TT2MPJJ7JCYHWAC0KJDD dateModified "2024-07-09T15:33:03Z".
- 01HQR1TT2MPJJ7JCYHWAC0KJDD name "Pulsed-electromagnetic field stimulation and mechanical stimulation for enhancing bovine myoblast myogenesis".
- 01HQR1TT2MPJJ7JCYHWAC0KJDD sameAs LU-01HQR1TT2MPJJ7JCYHWAC0KJDD.
- 01HQR1TT2MPJJ7JCYHWAC0KJDD sourceOrganization urn:uuid:1f22f495-9291-4631-8768-021a6196713b.
- 01HQR1TT2MPJJ7JCYHWAC0KJDD sourceOrganization urn:uuid:9a2473d9-3480-4ed5-bfd5-c135480db0bf.
- 01HQR1TT2MPJJ7JCYHWAC0KJDD type C3.