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- 01HS8JZENPVEMVE37VA54V0MEB classification C3.
- 01HS8JZENPVEMVE37VA54V0MEB date "2020".
- 01HS8JZENPVEMVE37VA54V0MEB language "eng".
- 01HS8JZENPVEMVE37VA54V0MEB type conference.
- 01HS8JZENPVEMVE37VA54V0MEB hasPart 01HS8K6T06JXXQ7KPD5X0QWZZ9.docx.
- 01HS8JZENPVEMVE37VA54V0MEB subject "Agriculture and Food Sciences".
- 01HS8JZENPVEMVE37VA54V0MEB subject "Biology and Life Sciences".
- 01HS8JZENPVEMVE37VA54V0MEB subject "Medicine and Health Sciences".
- 01HS8JZENPVEMVE37VA54V0MEB presentedAt urn:uuid:68578159-1139-48bf-9f2f-9fe23d1657f4.
- 01HS8JZENPVEMVE37VA54V0MEB abstract "Colorectal cancer remains one of the leading causes of cancer death in developed countries, even though it is estimated that one third of cases could be avoided by a change in lifestyle factors. In this context, consumption of red meat and processed meat has been correlated with a higher risk of developing colorectal cancer. However, the underlying mechanisms remain unclear. We have used established colon cancer cell line models in monolayer and spheroid configurations to collect indications of the involved pathways on a cellular level. Exposure to a selection of meat matrix/digestion compounds in sub-toxic concentrations resulted in a different behavior of treated vs. untreated cells when measuring proliferation and viability, and caused disturbances of the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), the colony forming capacity and wound healing capacity. These observations suggest a role of meat digestion compounds in colorectal carcinogenesis by a direct effect on cell metabolism. Our hypothesis is that red meat digestion products can stimulate key cellular pathways such as the PI3K- and Wnt-pathway, which we will further investigate. Although cell line models are useful for learning about mechanistic effects, their use has important limitations in terms of translation to the human body and has the disadvantage of being of cancerous origin. Therefore we are currently culturing small intestine and colon organoids from mouse models. We use intestinal crypts of healthy mice of different genetic backgrounds including wild type, Apcf and p53m. The impact of the presence of meat related compounds in the extracellular matrix is examined by adding the compound to the surrounding medium at the time of crypt seeding. To simulate the impact of diet in the body, we suggest a long-exposure-low-dose approach. Throughout the exposure experiment, the colon organoids are monitored in terms of growth and morphology, and at the endpoint immunohistochemistry, immunofluorescence, real time Seahorse XF analyses, western blot and metabolomics are performed. In this way we want to elucidate if diet-related compounds change the cellular pathways towards more malignant phenotypes. This will lead to a better understanding of the etiology of colorectal cancer and the role of diet herein.".
- 01HS8JZENPVEMVE37VA54V0MEB author 4B50209E-FC5C-11E1-8B8A-AC6710BDE39D.
- 01HS8JZENPVEMVE37VA54V0MEB author F4B233EA-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HS8JZENPVEMVE37VA54V0MEB author F5D0FA72-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HS8JZENPVEMVE37VA54V0MEB author F7A30E30-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HS8JZENPVEMVE37VA54V0MEB dateCreated "2024-03-18T10:51:06Z".
- 01HS8JZENPVEMVE37VA54V0MEB dateModified "2024-07-09T15:33:11Z".
- 01HS8JZENPVEMVE37VA54V0MEB name "Effect of (processed) meat related compounds on intestinal organoids".
- 01HS8JZENPVEMVE37VA54V0MEB sameAs LU-01HS8JZENPVEMVE37VA54V0MEB.
- 01HS8JZENPVEMVE37VA54V0MEB sourceOrganization urn:uuid:70bf8b6c-f384-483c-a898-8787b84379c0.
- 01HS8JZENPVEMVE37VA54V0MEB sourceOrganization urn:uuid:c4c5a3c5-edd2-4c60-9ab7-d6f999a259d3.
- 01HS8JZENPVEMVE37VA54V0MEB type C3.