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- 01HS8X0W8RVAQYPRRV80YWJ7T9 classification C3.
- 01HS8X0W8RVAQYPRRV80YWJ7T9 date "2022".
- 01HS8X0W8RVAQYPRRV80YWJ7T9 language "eng".
- 01HS8X0W8RVAQYPRRV80YWJ7T9 type conference.
- 01HS8X0W8RVAQYPRRV80YWJ7T9 hasPart 01HS8X2NWQFPPWJF9PR427RF9V.docx.
- 01HS8X0W8RVAQYPRRV80YWJ7T9 subject "Agriculture and Food Sciences".
- 01HS8X0W8RVAQYPRRV80YWJ7T9 subject "Medicine and Health Sciences".
- 01HS8X0W8RVAQYPRRV80YWJ7T9 presentedAt urn:uuid:d7a31b5f-571d-40d6-b39e-cd563b1e2906.
- 01HS8X0W8RVAQYPRRV80YWJ7T9 abstract "Reprogrammed cell metabolism is a cancer hallmarks [1] and involves the Warburg effect, glutamine addiction, increased dependence on one-carbon metabolism and fatty acid oxidation, and metabolic symbioses between different cell types [2-6]. The availability of substrates in the two-front nutritional colonic microenvironment, as well as chronic dietary habits, could affect the energetic options of CRC cells, reduce their metabolic flexibility, and make them more vulnerable. The aim of this study was to characterize the metabolic behavior of 3D CRC spheroids in different nutritional contexts. A long-term-sub-toxic-dose experimental set-up was developed to provide insights in (i) the chronic effects of luminal exposure to dietary and microbial metabolites, and (ii) the impact of media types with different nutrient-richness. Using extracellular flux analysis and RNA Illumina sequencing, we demonstrated that the access to glucose, glutamine, pyruvate and/or butyrate dictates the metabolic profile of CRC spheroids, who display high levels of both glycolysis and mitochondrial respiration. Moreover, the chronic pretreatment with metabolites including butyrate, hemin, kynurenine and kynurenic acid altered the metabolic flexibility, which allowed them to support both glycolysis and mitochondrial respiration or easily switch between them when specific substrates became limited. These results show that chronic exposure to dietary compounds can affect the metabolic plasticity of CRC spheroids, and underscore the importance of the medium composition, choice of cell line, and time of exposure in nutritional and metabolic research.".
- 01HS8X0W8RVAQYPRRV80YWJ7T9 author 4B50209E-FC5C-11E1-8B8A-AC6710BDE39D.
- 01HS8X0W8RVAQYPRRV80YWJ7T9 author F4B233EA-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HS8X0W8RVAQYPRRV80YWJ7T9 author F5D0FA72-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HS8X0W8RVAQYPRRV80YWJ7T9 author F6996D04-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HS8X0W8RVAQYPRRV80YWJ7T9 author F7A30E30-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HS8X0W8RVAQYPRRV80YWJ7T9 dateCreated "2024-03-18T13:46:38Z".
- 01HS8X0W8RVAQYPRRV80YWJ7T9 dateModified "2024-07-09T15:30:49Z".
- 01HS8X0W8RVAQYPRRV80YWJ7T9 name "Effect of chronic exposure of dietary compounds on colorectal cancer bioenergetics".
- 01HS8X0W8RVAQYPRRV80YWJ7T9 pagination urn:uuid:4f64d1e5-d0e7-4988-8d1e-89d4027cc1ee.
- 01HS8X0W8RVAQYPRRV80YWJ7T9 sameAs LU-01HS8X0W8RVAQYPRRV80YWJ7T9.
- 01HS8X0W8RVAQYPRRV80YWJ7T9 sourceOrganization urn:uuid:aeed4dc4-54fd-4089-8c48-949625eba21a.
- 01HS8X0W8RVAQYPRRV80YWJ7T9 sourceOrganization urn:uuid:d3b3841d-1253-40b2-ba17-177557d4237e.
- 01HS8X0W8RVAQYPRRV80YWJ7T9 type C3.