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- 01HSTHT16MJYKQXB0S6NWWZ4HW classification C3.
- 01HSTHT16MJYKQXB0S6NWWZ4HW date "2023".
- 01HSTHT16MJYKQXB0S6NWWZ4HW language "eng".
- 01HSTHT16MJYKQXB0S6NWWZ4HW type conference.
- 01HSTHT16MJYKQXB0S6NWWZ4HW hasPart 01HSTT3MNT8MRMTP7XDVNQ3PCH.pdf.
- 01HSTHT16MJYKQXB0S6NWWZ4HW subject "Biology and Life Sciences".
- 01HSTHT16MJYKQXB0S6NWWZ4HW subject "Medicine and Health Sciences".
- 01HSTHT16MJYKQXB0S6NWWZ4HW presentedAt urn:uuid:5985f015-e033-4998-8a52-e242c867da2a.
- 01HSTHT16MJYKQXB0S6NWWZ4HW abstract "Background Circulating tumor cells (CTCs) are cancer cells that enter the circulatory system and form distant metastases. CTCs are shown to be strongly associated with poor survival and therapy failure and thus present an opportunity to monitor disease. Parsortix is a size-based microfluidic system for CTC enrichment, which was recently FDA approved for CTC detection in metastatic breast cancer patients. The suitability of the Parsortix instrument for the isolation of pancreatic CTCs has not been previously investigated. The aim of our study is to determine the recovery rates of the Parsortix instrument for different pancreatic cancer cell subtypes. Methods Three pancreatic cancer cell lines were used in this study: CAPAN-1, PANC-1 and MIA-PaCa-2. An in-depth proteome analysis was performed using data-independent acquisition (DIA)-mass spectrometry (MS) to identify differentially expressed proteins. In addition, we stained each cell line with a different CellTracker dye and spiked them into blood from healthy donors. We processed the samples using the Parsortix system and determined the recovery rates by quantifying the number of cancer cells in the separation cassette using fluorescence microscopy. Results MS analysis identified a total of 6590 proteins in all cell lines. By evaluating levels of currently used protein markers for Epithelial Mesenchymal Transition (EMT) such as EpCAM, cytokeratin and vimentin, we were able to characterize CAPAN-1, MIA-PaCa-2, and PANC-1 as epithelial, mesenchymal and intermediate cells respectively. The three cell lines were fluorescently labeled for marker-independent detection in the Parsortix. Our preliminary results suggest that MIA-PaCa-2 (mesenchymal cells) may have a lower recovery rate (36%) with the Parsortix system compared to CAPAN-1 (epithelial cells) (41%). The overall recovery rates were also lower than expected compared to current literature. Additional experiments are ongoing to confirm these findings. Conclusions Overall, our study highlights the importance of evaluating the recovery rates of different CTC subtypes to develop more effective methods for capturing the heterogeneity of CTCs in liquid biopsies. Further experiments are needed to confirm the Parsortix system's ability to enrich mesenchymal CTCs as effectively as epithelial CTCs in patients with pancreatic cancer.".
- 01HSTHT16MJYKQXB0S6NWWZ4HW author 1A8A0462-F0EE-11E1-A9DE-61C894A0A6B4.
- 01HSTHT16MJYKQXB0S6NWWZ4HW author 2B4C1E7A-F0EE-11E1-A9DE-61C894A0A6B4.
- 01HSTHT16MJYKQXB0S6NWWZ4HW author 51B88C8A-600F-11E6-BB46-0867B5D1D7B1.
- 01HSTHT16MJYKQXB0S6NWWZ4HW author CA810F26-BED4-11E4-A4DF-4E77B5D1D7B1.
- 01HSTHT16MJYKQXB0S6NWWZ4HW author E1FD4698-D2A5-11E5-B168-7440B5D1D7B1.
- 01HSTHT16MJYKQXB0S6NWWZ4HW author F4EC703C-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HSTHT16MJYKQXB0S6NWWZ4HW author F525876E-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HSTHT16MJYKQXB0S6NWWZ4HW author ffcc303c-333e-11ef-b5a5-d838e7d63346.
- 01HSTHT16MJYKQXB0S6NWWZ4HW dateCreated "2024-03-25T10:16:59Z".
- 01HSTHT16MJYKQXB0S6NWWZ4HW dateModified "2024-11-27T23:59:44Z".
- 01HSTHT16MJYKQXB0S6NWWZ4HW name "Assessing recovery rates of mesenchymal and epithelial pancreatic tumor cells using the Parsortix system".
- 01HSTHT16MJYKQXB0S6NWWZ4HW sameAs LU-01HSTHT16MJYKQXB0S6NWWZ4HW.
- 01HSTHT16MJYKQXB0S6NWWZ4HW sourceOrganization urn:uuid:5e33b368-56b7-42bd-869f-a05dd10c79f2.
- 01HSTHT16MJYKQXB0S6NWWZ4HW sourceOrganization urn:uuid:ab7249b3-9887-468a-9969-12eadb969137.
- 01HSTHT16MJYKQXB0S6NWWZ4HW sourceOrganization urn:uuid:d73dcd32-f5a7-46f2-bd71-944f8b4b01ee.
- 01HSTHT16MJYKQXB0S6NWWZ4HW type C3.