Matches in Ghent University Academic Bibliography for { <https://biblio.ugent.be/publication/01JB9EFMWM7TMH0AR3EGS18CBH> ?p ?o. }
Showing items 1 to 21 of
21
with 100 items per page.
- 01JB9EFMWM7TMH0AR3EGS18CBH classification C3.
- 01JB9EFMWM7TMH0AR3EGS18CBH date "2024".
- 01JB9EFMWM7TMH0AR3EGS18CBH language "eng".
- 01JB9EFMWM7TMH0AR3EGS18CBH type conference.
- 01JB9EFMWM7TMH0AR3EGS18CBH hasPart 01JB9F946NSGQHF3QV6T4FVBFR.docx.
- 01JB9EFMWM7TMH0AR3EGS18CBH subject "Veterinary Sciences".
- 01JB9EFMWM7TMH0AR3EGS18CBH presentedAt urn:uuid:aa763b75-883e-47dd-9576-78dfb6b94489.
- 01JB9EFMWM7TMH0AR3EGS18CBH abstract "Type 2 diabetes, a metabolic disorder characterized by impaired insulin secretion and action, has currently reached the scale of a global pandemic. It remains one of the leading causes of blindness, end-stage renal disease, lower limb amputation, and cardiovascular disease. Similar to diabetes in humans, horses can suffer from equine metabolic syndrome (EMS), which is also associated with obesity, insulin dysregulation, and vascular wall changes. A suitable in vitro vascular wall model for drug screening is needed to improve the treatment of both equine and human patients. The ideal vascular tissue engineering structure must generally resemble all mechanical and biological properties similar to native vessels while being non-thrombogenic and non-immunogenic, making the choice of material crucial. Polymers made from acrylate-endcapped urethane-based polymer precursor (AUP) with a polyethylene glycol (PEG) backbone, called AUPPEGs, combine the characteristics of polyurethanes and PEG, and the polymer properties can be relatively easily tuned. Therefore, the present study aimed to evaluate the effects of coating AUPPEG scaffolds manufactured via digital light processing (DLP) with collagen or gelatine derivatives on their cell-interactive properties towards equine adipose-derived mesenchymal stem cells (eqAT-MSCs) and endothelial cells (eqECs). Diacrylate end-capped urethane-based PEG (AUP2PEG) was synthesized via a 2-step modification of PEG (2000 g mol-1) as reported previously. The 3D scaffolds were fabricated using DLP printing (resin solution: 30 wt% AUP2PEG, 10 mol% LiTPO-L, 1 mol% tartrazine, solvent: ultra-pure water; printing parameters: irradiation time 4 s; 23.72 mW/cm2 light intensity). The surface of the scaffolds was coated with gelatin methacryloyl (GelMA) or bovine atelocollagen. The GelMA coating was applied exploiting a protocol described earlier. The atelocollagen coating was applied by immersion of Argon plasma-activated scaffolds (0.5 min at pressure 0.8 mbar, and a power 100 W, after the plasma treatment, the scaffolds were exposed to the ambient atmosphere for 20 min) in a bovine atelocollagen type I solution (1 mg/mL in 0.01 M acetic acid, without subsequent chemical crosslinking). The eqAT-MSC and eqEC, isolated using enzymatic digestion techniques, were seeded onto the scaffolds at a density of 20,000 cells/ cm2 or 10,000 cells/cm2, respectively. A calcein-AM/PI staining was performed to evaluate the viability of the cells. The ability of the eqAD-MSC to differentiate adipogenically and osteogenically on the scaffolds was assessed as described earlier. Both GelMA and atelocollagen coatings improved the short-term (up to 7 days post-seeding) cytocompatibility of DLP-printed AUP2PEG-based scaffolds for eqECs and eqAD-MSCs compared to the uncoated scaffolds. However, during further cultivation (up to 21 days post-seeding), partially adherent spheroid-like aggregates of the eqECs and eqAD-MSCs were detected on the atelocollagen-coated scaffolds. Considering that the cells remained viable, the observed phenomena could be due to the detachment of the coating from the surface. Both GelMA and atelocollagen coating supported adipogenic and osteogenic differentiation of eqAD-MSCs. To mimic diabetic/EMS conditions, the GelMA-coated AUP2PEG scaffolds were incubated in a high glucose medium (35 mM glucose). Short-term cultivation (72 hours) of eqECs in a high glucose medium significantly reduced the number of viable cells attached to the surface compared to cultivation in a low glucose medium (5 mM glucose). In conclusion, our results indicate that GelMA-coated DLP-printed AUP2PEG scaffolds support eqEC and eqAD-MSC cultures.".
- 01JB9EFMWM7TMH0AR3EGS18CBH author 32481fb2-f340-11ec-8c23-b6a6773ba13e.
- 01JB9EFMWM7TMH0AR3EGS18CBH author 3C783F08-F0EE-11E1-A9DE-61C894A0A6B4.
- 01JB9EFMWM7TMH0AR3EGS18CBH author 99ED24CC-1E7E-11E4-B0FC-3B54B5D1D7B1.
- 01JB9EFMWM7TMH0AR3EGS18CBH author F6EFF23C-F0ED-11E1-A9DE-61C894A0A6B4.
- 01JB9EFMWM7TMH0AR3EGS18CBH author F7AF8B88-F0ED-11E1-A9DE-61C894A0A6B4.
- 01JB9EFMWM7TMH0AR3EGS18CBH dateCreated "2024-10-28T11:34:21Z".
- 01JB9EFMWM7TMH0AR3EGS18CBH dateModified "2024-12-12T21:08:36Z".
- 01JB9EFMWM7TMH0AR3EGS18CBH name "Digital light processing of photo-crosslinkable hydrogels towards an equine vascular wall model.".
- 01JB9EFMWM7TMH0AR3EGS18CBH pagination urn:uuid:0e10d836-7364-4455-8011-119420520a56.
- 01JB9EFMWM7TMH0AR3EGS18CBH sameAs LU-01JB9EFMWM7TMH0AR3EGS18CBH.
- 01JB9EFMWM7TMH0AR3EGS18CBH sourceOrganization urn:uuid:77d8a242-275c-46c8-8360-05035a37a87f.
- 01JB9EFMWM7TMH0AR3EGS18CBH sourceOrganization urn:uuid:cce76c46-9d17-4d4d-844a-73ddd34db398.
- 01JB9EFMWM7TMH0AR3EGS18CBH type C3.