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- aggregation classification "C3".
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2010".
- aggregation format "application/pdf".
- aggregation hasFormat 1026203.bibtex.
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- aggregation isPartOf urn:issn:0936-6768.
- aggregation language "eng".
- aggregation publisher "WILEY-BLACKWELL PUBLISHING, INC".
- aggregation rights "I don't know the status of the copyright for this publication".
- aggregation subject "Veterinary Sciences".
- aggregation title "Influence of sperm-oocyte coincubation period on porcine in vitro fertilization (IVF) efficiency".
- aggregation abstract "A major obstacle for successful in vitro production of porcine embryos is the polyspermic fertilization. One possibility to reduce polyspermic penetration is decreasing the number of spermatozoa added to the fertilization medium. Unfortunately, the lower rate of polyspermy is accompanied by a reduced penetration rate. A short gamete coincubation period of 10 min has been described to obtain fertilization rates similar to 6 h of coincubation and may improve IVF efficiency (number of monospermic fertilized oocytes/total number inseminated) depending on sperm-oocyte ratio (Gil, 2007, Theriogenology, 67(3), 620–626). Here we demonstrate that the optimal coincubation period in our IVF conditions is between 10 min and 6 h. In vitro matured oocytes (n = 600) were inseminated with frozen-thawed epididymal semen with 600 spermatozoa per oocyte and coincubated for 2, 4 and 6 h. At 2 and 4 h post insemination (hpi), oocytes were vortexed and transferred to fertilization medium without spermatozoa. At 6 hpi, presumed zygotes of all groups were washed three times in culture medium and cultured. At 22 hpi, zygotes were fixed overnight and stained with Hoechst 33,342 for the assessment of fertilization and polyspermy. The IVF efficiency was higher for the 4 h group (40 ± 5%) than the 2 and 6 h group (19 ± 8% and 17 ± 5%). Between 4 and 6 h of gamete coincubation, the increase in the number of polyspermic oocytes was relatively higher than the increase in penetration rate (+39% vs. +15%), resulting in a decline in efficiency. (This study was supported by Research Foundation-Flanders).".
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- aggregation endPage "78".
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- aggregation volume "45".
- aggregation aggregates 1026575.
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