Matches in UGent Biblio for { <https://biblio.ugent.be/publication/1089037#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B582518.
- aggregation creator B582519.
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- aggregation creator person.
- aggregation creator person.
- aggregation date "2010".
- aggregation format "application/pdf".
- aggregation hasFormat 1089037.bibtex.
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- aggregation isPartOf urn:issn:1431-9276.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Science General".
- aggregation title "Colocalization analysis in fluorescence micrographs: verification of a more accurate calculation of Pearson's correlation coefficient".
- aggregation abstract "One of the most routine uses of fluorescence microscopy is colocalization, i.e., the demonstration of a relationship between pairs of biological molecules. Frequently this is presented simplistically by the use of overlays of red and green images, with areas of yellow indicating colocalization of the molecules. Colocalization data are rarely quantified and can be misleading. Our results from both synthetic and biological datasets demonstrate that the generation of Pearson's correlation coefficient between pairs of images can overestimate positive correlation and fail to demonstrate negative correlation. We have demonstrated that the calculation of a thresholded Pearson's correlation coefficient using only intensity values over a determined threshold in both channels produces numerical values that more accurately describe both synthetic datasets and biological examples. Its use will bring clarity and accuracy to colocalization studies using fluorescent microscopy.".
- aggregation authorList BK934414.
- aggregation endPage "724".
- aggregation issue "6".
- aggregation startPage "710".
- aggregation volume "16".
- aggregation aggregates 2937634.
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